Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi - 110067, India.
Proteome Sci. 2011 Feb 4;9:5. doi: 10.1186/1477-5956-9-5.
Hepatitis E is endemic to resource-poor regions, where it manifests as sporadic cases and large waterborne outbreaks. The disease severity ranges from acute self-limited hepatitis with low mortality to fulminant hepatic failure with high mortality. It is believed that the host response plays an important role in determining the progression and outcome of this disease. We profiled the plasma peptidome from hepatitis E patients to discover suitable biomarkers and understand disease pathogenesis.
The peptidome (< 10 kDa) fraction of plasma was enriched and analyzed by mass spectrometry. A comparative analysis of the peptide pattern of hepatitis E patients versus healthy controls was performed using ClinPro Tools. We generated a peptide profile that could be used for selective identification of hepatitis E cases. We have identified five potential biomarker peaks with m/z values of 9288.6, 7763.6, 4961.5, 1060.572 and 2365.139 that can be used to reliably differentiate between hepatitis E patients and controls with areas under the receiver operating characteristic curve (AUROC) values of 1.00, 0.954, 0.989, 0.960 and 0.829 respectively. A number of proteins involved in innate immunity were identified to be differentially present in the plasma of patients compared to healthy controls.
Besides the utility of this approach for biomarker discovery, identification of changes in endogenous peptides in hepatitis E patient plasma has increased our understanding of disease pathogenesis. We have identified peptides in plasma that can reliably distinguish hepatitis E patients from healthy controls. Results from this and an earlier proteomics study are discussed.
戊型肝炎在资源匮乏地区流行，表现为散发性病例和大规模水传播暴发。疾病严重程度从死亡率低的急性自限性肝炎到死亡率高的暴发性肝衰竭不等。据信，宿主反应在决定该病的进展和结局方面起着重要作用。我们对戊型肝炎患者的血浆肽组进行了分析，以发现合适的生物标志物并了解疾病发病机制。
通过质谱法对血浆的肽组（<10 kDa）部分进行了富集和分析。使用 ClinPro Tools 对戊型肝炎患者与健康对照者的肽图谱进行了比较分析。我们生成了一个可用于选择性识别戊型肝炎病例的肽谱。我们已经确定了五个潜在的生物标志物峰，其 m/z 值分别为 9288.6、7763.6、4961.5、1060.572 和 2365.139，其 AUC 值分别为 1.00、0.954、0.989、0.960 和 0.829，可用于可靠地区分戊型肝炎患者和对照者。与健康对照者相比，在患者的血浆中鉴定出许多参与固有免疫的蛋白质存在差异。
除了该方法在生物标志物发现方面的应用外，鉴定戊型肝炎患者血浆中内源性肽的变化增加了我们对发病机制的理解。我们已经鉴定出可可靠地区分戊型肝炎患者和健康对照者的血浆肽。讨论了本研究和早期蛋白质组学研究的结果。
