Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, 24 Tongjiaxiang, Nanjing 210009, People's Republic of China.
Toxicology. 2011 Apr 11;282(3):122-8. doi: 10.1016/j.tox.2011.01.018. Epub 2011 Feb 2.
Wogonin, a naturally occurring monoflavonoid extracted from Scutellariae radix, has been shown to possess tumor therapeutic potential in vitro and in vivo. However, the effects of wogonin on tumor cells invasion remains poorly understood. In this study, we performed in vitro experiments to investigate the anti-invasive and anti-metastatic activity of wogonin in MDA-MB-231 human breast carcinoma cells. Wogonin caused a concentration-dependent suppression of cell migration, adhesion and invasion. The mechanism revealed that wogonin significantly inhibited the expression and activity of both endogenous and phorbol-12-myristate-13-acetate (PMA)-induced matrix metalloproteinase-9 (MMP-9) potentially associating with the suppression of translocation of protein kinase C (PKC) δ and phosphorylation of extracellular signal-regulated kinase (ERK1/2). These results suggested that wogonin could inhibit the invasion of tumor cells by downregulating the expression and activity of MMP-9, the possible targets may be PKCδ and ERK1/2.
汉黄芩素是一种从黄芩根中提取的天然单黄酮类化合物，已被证明具有体外和体内的肿瘤治疗潜力。然而，汉黄芩素对肿瘤细胞侵袭的影响仍知之甚少。在这项研究中，我们进行了体外实验，以研究汉黄芩素对 MDA-MB-231 人乳腺癌细胞的抗侵袭和抗转移活性。汉黄芩素呈浓度依赖性抑制细胞迁移、黏附和侵袭。机制研究表明，汉黄芩素显著抑制内源性和佛波醇 12-肉豆蔻酸 13-乙酸酯（PMA）诱导的基质金属蛋白酶-9（MMP-9）的表达和活性，可能与蛋白激酶 C（PKC）δ易位和细胞外信号调节激酶（ERK1/2）磷酸化的抑制有关。这些结果表明，汉黄芩素可以通过下调 MMP-9 的表达和活性来抑制肿瘤细胞的侵袭，其可能的靶点是 PKCδ 和 ERK1/2。
