血清 miR-221 在人肝细胞癌中的表达及其预后意义。

Expression of serum miR-221 in human hepatocellular carcinoma and its prognostic significance.

机构信息

Laboratory Medicine Center, Yinzhou People's Hospital, Ningbo 315040, China.

出版信息

Biochem Biophys Res Commun. 2011 Mar 4;406(1):70-3. doi: 10.1016/j.bbrc.2011.01.111. Epub 2011 Feb 3.

DOI:10.1016/j.bbrc.2011.01.111

PMID:21295551

Abstract

OBJECTIVE

To investigate whether the serum miR-221 expression correlates with clinicopathologic features and the prognosis of hepatocellular carcinoma (HCC) patients.

METHODS

Four miRNAs (miR-221, miR-222, miR-21 and miR-224) related to HCC were selected in the present study. Serum miRNA expression was investigated in 46 HCC patients and 20 healthy normal controls by using real-time PCR technique, and then correlations between miR-221 expression and the clinicopathological features and prognosis of HCC patients were evaluated.

RESULTS

The four miRNAs were found to be differentially overexpressed in HCC serum samples, and high level of miR-221 expression was correlated with tumor size (P<0.001), cirrhosis (P=0.003) and tumor stage (P=0.016). In addition, Kaplan-Meier survival analysis showed that the overall survival rate of the high miR-221 expression group (27.6%) was significantly lower than that of the low miR-221 expression group (62.3%, P<0.05).

CONCLUSIONS

Serum miR-221, upregulated in HCC, can provide predictive significance for prognosis of HCC patients.

摘要

目的

探讨血清 miR-221 表达与肝细胞癌（HCC）患者临床病理特征及预后的关系。

方法

本研究选择了与 HCC 相关的 4 种 miRNA（miR-221、miR-222、miR-21 和 miR-224）。采用实时 PCR 技术检测 46 例 HCC 患者和 20 例健康正常对照者血清 miRNA 表达，评估 miR-221 表达与 HCC 患者临床病理特征及预后的相关性。

结果

发现这 4 种 miRNA 在 HCC 血清样本中存在差异表达，miR-221 高表达与肿瘤大小（P<0.001）、肝硬化（P=0.003）和肿瘤分期（P=0.016）有关。此外，Kaplan-Meier 生存分析显示，miR-221 高表达组（27.6%）的总生存率明显低于 miR-221 低表达组（62.3%，P<0.05）。