Department of Farm Animal Health, Faculty of Veterinary Medicine, Utrecht, The Netherlands.
Theriogenology. 2011 Apr 15;75(7):1360-70. doi: 10.1016/j.theriogenology.2010.11.046. Epub 2011 Feb 4.
The objective was to compare sperm characteristics between the two subspecies of North American bison, plains bison (Bison bison bison) and wood bison (Bison bison athabascae). Frozen-thawed ejaculated sperm from age-matched plains (n = 3) and wood (n = 2) bison were evaluated for morphometry, motility, viability, protein profile, and in vitro fertilization characteristics. Sperm morphometry and motility were assessed with computer-based systems, viability was assessed with SYBR-14 and propidium iodide, and fertilizing ability was determined using a heterologous in vitro fertilization system (using bovine oocytes). For plains versus wood bison, there were significant differences for head width (4.76 ± 0.22 vs 4.71 ± 0.19 μm; mean ± SD), head area (35.64 ± 1.91 vs 34.72 ± 2.64 μm(2)), head perimeter (23.61 ± 0.68 vs 23.31 ± 0.98 μm), midpiece length (14.58 ± 0.4 vs 14.36 ± 0.51 μm), midpiece width (0.81 ± 0.06 vs 0.79 ± 0.07 μm), and tail length (46.61 ± 2.15 vs 45.98 ± 2.08 μm). However, there was no significant difference in head length (overall, 9.04 ± 0.37 μm), progressive motility (41.16 ± 8.39%), or viability (41.58 ± 5.58%). Based on two-dimensional gel electrophoresis, 93 out of 113 protein spots were similar in their expression patterns. Furthermore, we inferred that differences in sperm biometry between these subspecies did not affect in vitro fertilization percentage (overall, 82.62 ± 12.13%). Based on these findings, we concluded that plains bison were an appropriate research model for developing reproductive technologies for wood bison.
本研究旨在比较北美野牛的两个亚种——平原野牛（Bison bison bison）和伍德野牛（Bison bison athabascae）的精子特征。对年龄匹配的平原野牛（n=3）和伍德野牛（n=2）的冷冻解冻精液进行形态学、运动性、活力、蛋白质谱和体外受精特征评估。使用基于计算机的系统评估精子形态学和运动性，使用 SYBR-14 和碘化丙啶评估活力，使用异种体外受精系统（使用牛卵母细胞）确定受精能力。对于平原野牛和伍德野牛，头宽（4.76±0.22 与 4.71±0.19 μm；平均值±标准差）、头面积（35.64±1.91 与 34.72±2.64 μm(2)）、头周长（23.61±0.68 与 23.31±0.98 μm）、中段长度（14.58±0.4 与 14.36±0.51 μm）、中段宽度（0.81±0.06 与 0.79±0.07 μm）和尾部长度（46.61±2.15 与 45.98±2.08 μm）存在显著差异。然而，头部长（总体，9.04±0.37 μm）、前向运动性（41.16±8.39%）或活力（41.58±5.58%）没有显著差异。基于二维凝胶电泳，113 个蛋白点中有 93 个在表达模式上相似。此外，我们推断这些亚种之间的精子生物计量学差异不会影响体外受精率（总体，82.62±12.13%）。基于这些发现，我们得出结论，平原野牛是开发伍德野牛生殖技术的合适研究模型。
