Environmental Genomics Division, National Environmental Engineering Research Institute (NEERI), CSIR, Nehru Marg, Nagpur 440 020, MH, India.
Bioresour Technol. 2011 Apr;102(7):4600-9. doi: 10.1016/j.biortech.2011.01.011. Epub 2011 Jan 9.
Genetic and functional diversity of Citrobacter spp. for their abilities to degrade aromatic compounds was evaluated to develop mixed cultures or a consortium for bioremediation technology. Thirty Citrobacter strains isolated from various effluent treatment plants were found to degrade a range of aromatic compounds: phenol, benzoate, hydroxy benzoic acid and biotransform mono-chlorophenols and di-chlorophenol within 24 to 48 h of incubation at 30 °C. Sequence similarity and phylogeny of the ARHD gene transcripts (730 nucleotides) depicted their diversity within 9 Citrobacter strains: HPC255, HPC369, HPC560, HPC570, HPC784, HPC1196, HPC1216, HPC1276 and HPC1299. Here, the degree of associations varied up to 84% with (i) ARHD α-sub unit (SU), (ii) LSU of Phenylpropionate dioxygenase (PDO), (iii) Phenol hydroxylase α-SU, (iv) Benzoate 1,2-dioxygenase, α-SU, (v) Naphthalene dioxygenase LSU, etc. This study has provided basic information, which can be used to develop a consortium of bacteria with mutually beneficial characteristics.
为了开发用于生物修复技术的混合培养物或生物群落，评估了柠檬酸杆菌属（Citrobacter spp.）的遗传和功能多样性，以了解其降解芳香族化合物的能力。从各种废水处理厂中分离出的 30 株柠檬酸杆菌被发现能够在 30°C 下孵育 24 至 48 小时内降解一系列芳香族化合物：苯酚、苯甲酸、羟基苯甲酸以及单氯酚和二氯酚的生物转化。ARHD 基因转录本（730 个核苷酸）的序列相似性和系统发育表明，9 株柠檬酸杆菌属（Citrobacter）内的多样性：HPC255、HPC369、HPC560、HPC570、HPC784、HPC1196、HPC1216、HPC1276 和 HPC1299。在此，关联度的差异高达 84%，包括 (i) ARHD α-亚基（SU）、(ii) 苯丙氨酸双加氧酶（PDO）的 LSU、(iii) 苯酚羟化酶 α-SU、(iv) 苯甲酸 1,2-双加氧酶，α-SU、(v) 萘二氧合酶 LSU 等。本研究提供了基本信息，可用于开发具有互利特性的细菌生物群落。
