State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, People's Republic of China.
Bioresour Technol. 2011 Apr;102(7):4595-9. doi: 10.1016/j.biortech.2011.01.003. Epub 2011 Jan 7.
Optically active D-2-hydroxybutanoate is an important building block intermediate for medicines and biodegradable poly(2-hydroxybutanoate). Kinetic resolution of racemic 2-hydroxybutanoate may be a green and desirable alternative for D-2-hydroxybutanoate production. In this work, D-2-hydroxybutanoate at a high concentration (0.197 M) and a high enantiomeric excess (99.1%) was produced by an NAD-independent L-lactate dehydrogenase (L-iLDH) containing biocatalyst. 2-Oxobutanoate, another important intermediate, was co-produced at a high concentration (0.193 M). Using a simple ion exchange process with the macroporous anion exchange resin D301, D-2-hydroxybutanoate was separated from the biotransformation system with a high recovery of 84.7%.
光学活性 D-2-羟基丁酸酯是一种重要的药物和可生物降解的聚（2-羟基丁酸酯）的结构单元中间体。消旋 2-羟基丁酸酯的动力学拆分可能是 D-2-羟基丁酸酯生产的一种绿色和理想的替代方法。在这项工作中，含有 NAD 非依赖性 L-乳酸脱氢酶（L-iLDH）的生物催化剂可将高浓度（0.197 M）和高对映体过量（99.1%）的 D-2-羟基丁酸酯转化为产物。同时，另一种重要的中间体 2-氧代丁酸也以高浓度（0.193 M）共生产出来。使用大孔阴离子交换树脂 D301 进行简单的离子交换过程，D-2-羟基丁酸酯可以从生物转化体系中以 84.7%的高回收率分离出来。
