Division of Cardiology, Department of Medicine, Taipei Veterans General Hospital and School of Medicine, National Yang-Ming University, Taipei, Taiwan.
J Vasc Surg. 2011 May;53(5):1189-94. doi: 10.1016/j.jvs.2010.10.122. Epub 2011 Feb 5.
Inflammation is associated with the disruption of the aortic media and appears to play a fundamental role in the progression and development of abdominal aortic aneurysm (AAA). Haptoglobin (Hp) is a genetically determined acute phase protein, the synthesis of which is increased during inflammation. This study was designed to investigate both phenotype and plasma levels of Hp in patients with AAA.
Patients with documented AAA who were admitted for elective open repair operation or endograft stent implantation, and non-AAA subjects admitted for coronary arteriography, but found to have normal or insignificant coronary artery disease, were included in the study. Plasma Hp levels were determined using a standard specific enzyme-linked immunosorbent assay, while Hp phenotype was determined by native polyacrylamide gel electrophoresis. Total cholesterol, high density lipoprotein, low density lipoprotein, and triglyceride levels were analyzed enzymatically, and C-reactive protein was analyzed by immunochemistry.
Forty-five patients with AAA and 49 non-AAA subjects were included. The Hp 2-2 phenotype was more predominant in AAA patients compared with non-AAA subjects, but this difference was not significant (67% vs 47%; P = .141), while plasma Hp concentrations were significantly higher in AAA patients (237 ± 144 vs 163 ± 86 ng/mL; P = .024). Further analysis revealed that plasma Hp concentrations were significantly higher in AAA patients with the 2-2 phenotype compared with corresponding non-AAA subjects (238 ± 144 vs 163 ± 86 ng/mL;P = .024).
Our findings suggest that plasma Hp concentrations are elevated in patients with AAA, particularly those with the Hp 2-2 phenotype.
炎症与主动脉中层的破坏有关，似乎在腹主动脉瘤（AAA）的进展和发展中起基本作用。触珠蛋白（Hp）是一种遗传决定的急性期蛋白，其合成在炎症期间增加。本研究旨在研究 AAA 患者的 Hp 表型和血浆水平。
本研究纳入了因择期开放修复手术或血管内支架植入术而入院的确诊 AAA 患者，以及因冠状动脉造影而入院但发现正常或无明显冠状动脉疾病的非 AAA 患者。使用标准的特异性酶联免疫吸附试验测定血浆 Hp 水平，并用天然聚丙烯酰胺凝胶电泳测定 Hp 表型。总胆固醇、高密度脂蛋白、低密度脂蛋白和甘油三酯水平用酶法分析，C-反应蛋白用免疫化学法分析。
本研究纳入了 45 例 AAA 患者和 49 例非 AAA 患者。与非 AAA 患者相比，AAA 患者的 Hp 2-2 表型更为常见，但差异无统计学意义（67%比 47%；P=.141），而 AAA 患者的血浆 Hp 浓度明显较高（237±144比 163±86 ng/ml；P=.024）。进一步分析显示，与相应的非 AAA 患者相比，具有 Hp 2-2 表型的 AAA 患者的血浆 Hp 浓度明显更高（238±144比 163±86 ng/ml；P=.024）。
我们的研究结果表明，AAA 患者的血浆 Hp 浓度升高，尤其是具有 Hp 2-2 表型的患者。
