Institute for Amphibian Biology, Graduate School of Science, Hiroshima University, Higashihiroshima 739-8526, Japan.
Mol Phylogenet Evol. 2011 Apr;59(1):139-47. doi: 10.1016/j.ympev.2011.01.015. Epub 2011 Feb 3.
In this study we sought to clarify the evolutionary relationships and biogeographic history of the bell-ring frog, Buergeria buergeri (family Rhacophoridae), and two congeneric species Buergeria japonica and Buergeria robusta, by analyzing three mitochondrial (mt) genes: 12S rRNA, Cytb, and ND5. Phylogenetic analyses based on gene data showed the mt clades corresponding to the Buergeria species and three major haplogroups within B. buergeri. Each haplogroup corresponded clearly to the area in which it was distributed, namely eastern Japan (excluding Hokkaido; Hg I), central Japan (Hg II), and western Japan (including the Shikoku and Kyushu regions; Hg III). The estimated divergence time suggested that the lineage splits of the Buergeria species occurred before the formation of the island of Taiwan and the Japan Archipelago (including the Ryukyu islands). The differentiation among the genealogical lineages of B. buergeri seems to have begun in the Late Miocene (approx. 7-5Mya), and the formation of their present distribution pattern might have been influenced by climatic changes and geographical events such as the formation of a wide peneplane and expansions of certain basins.
在这项研究中，我们通过分析三种线粒体（mt）基因：12S rRNA、Cytb 和 ND5，旨在阐明铃蟾属（Buergeria）的铃蟾（Buergeria buergeri，隶属于雨蛙科 Rhacophoridae）以及两个同属物种斑腿泛树蛙（Buergeria japonica）和粗壮泛树蛙（Buergeria robusta）的进化关系和生物地理历史。基于基因数据的系统发育分析显示，mt 支系与铃蟾属物种以及 B. buergeri 内的三个主要单倍群相对应。每个单倍群都与分布区域明确对应，即日本东部（不包括北海道；Hg I）、日本中部（Hg II）和日本西部（包括四国和九州地区；Hg III）。估计的分化时间表明，铃蟾属物种的谱系分裂发生在台湾岛和日本列岛（包括琉球群岛）形成之前。B. buergeri 种系的遗传分化似乎始于中新世晚期（约 7-5Mya），其目前分布格局的形成可能受到气候变化和地理事件的影响，如广泛的准平原形成和某些盆地的扩张。
