INSERM UMR 977, Faculté de Médecine, 11, rue Humann, F-67085 Strasbourg, France.
J Dent Res. 2011 May;90(5):583-9. doi: 10.1177/0022034510391796. Epub 2011 Feb 4.
Embryonic dental cells were used to check a series of criteria to be achieved for tooth engineering. Implantation of cultured cell-cell re-associations led to crown morphogenesis, epithelial histogenesis, organ vascularization, and root and periodontium development. The present work aimed to investigate the organization of predentin/dentin, enamel, and cementum which formed and mineralized after implantation. These implants were processed for histology, transmission electron microscopy, x-ray microanalysis, and electron diffraction. After two weeks of implantation, the re-associations showed gradients of differentiating odontoblasts. There were ciliated, polarized, and extended cell processes in predentin/dentin. Ameloblasts became functional. Enamel crystals showed a typical oriented arrangement in the inner and outer enamel. In the developing root, odontoblasts differentiated, cementogenesis occurred, and periodontal ligament fibroblasts interacted with the root surface and newly formed bone. The implantation of cultured dental cell re-associations allows for reproduction of complete functional differentiation at the cell, matrix, and mineral levels.
胚胎牙齿细胞被用于检查一系列牙工程学所需要达到的标准。培养的细胞-细胞重新组合的植入导致了牙冠形态发生、上皮组织发生、器官血管化以及牙根和牙周组织的发育。本研究旨在探讨植入后形成和矿化的前期牙本质/牙本质、釉质和牙骨质的组织。这些植入物经过组织学、透射电子显微镜、X 射线微分析和电子衍射处理。植入两周后，重新组合显示出分化成牙本质细胞的梯度。在前期牙本质/牙本质中有纤毛、极化和延伸的细胞突起。成釉细胞变得具有功能。釉质晶体在内外釉质中呈现出典型的有序排列。在发育中的牙根中，成牙本质细胞分化，牙骨质发生，牙周韧带成纤维细胞与牙根表面和新形成的骨相互作用。培养的牙细胞重新组合的植入允许在细胞、基质和矿物质水平上复制完整的功能分化。
