自身免疫性疾病中针对聚（ADP - 核糖）聚合酶的自身抗体。
Autoantibodies to poly(ADP-ribose)polymerase in autoimmune diseases.
作者信息
Negri C, Scovassi A I, Cerino A, Negroni M, Borzì R M, Meliconi R, Facchini A, Montecucco C M, Astaldi Ricotti G C
机构信息
Instituto di Genetica Biochimica ed Evoluzionistica del C.N.R., Pavia, Italy.
出版信息
Autoimmunity. 1990;6(3):203-9. doi: 10.3109/08916939009041040.
Antibodies to various nuclear proteins are frequently found in sera of patients affected by connective tissue diseases and other autoimmune diseases. We investigated the specificity of circulating autoantibodies to poly(ADP-ribose)polymerase (pADPRP) in different autoimmune and connective tissue diseases: Systemic Lupus Erythematosus (SLE), Rheumatoid Arthritis (RA), Progressive Systemic Sclerosis (PSS), Sjogren's Syndrome (SS), Undifferentiated Connective Tissue Disease (UCTD), Cryptogenic Fibrosing Alveolitis (CFA) and Sarcoidosis. pADPRP was purified from calf thymus. Antibody specificity was detected by ELISA, western blot and enzyme activity precipitation. Positive values (mean O.D. values + 3 S.D. of 36 normal controls) were obtained in 7/15 SLE patients, 1/18 RA patients, 1/30 PSS, 3/14 SS, 0/5 UCTD, 5/21 CFA and 4/25 Sarcoidosis. The positive sera also recognized the pADPRP protein when tested by western blot. Furthermore the enzyme activity was inhibited after immunoprecipitation by some highly positive sera.
在患有结缔组织疾病和其他自身免疫性疾病的患者血清中，经常能发现针对各种核蛋白的抗体。我们研究了不同自身免疫性疾病和结缔组织疾病中循环抗聚（ADP - 核糖）聚合酶（pADPRP）自身抗体的特异性，这些疾病包括系统性红斑狼疮（SLE）、类风湿性关节炎（RA）、进行性系统性硬化症（PSS）、干燥综合征（SS）、未分化结缔组织病（UCTD）、隐源性纤维性肺泡炎（CFA）和结节病。pADPRP从小牛胸腺中纯化得到。通过酶联免疫吸附测定（ELISA）、蛋白质印迹法（western blot）和酶活性沉淀法检测抗体特异性。在15例SLE患者中有7例、18例RA患者中有1例、30例PSS患者中有1例、14例SS患者中有3例、5例UCTD患者中无、21例CFA患者中有5例以及25例结节病患者中有4例获得阳性值（36名正常对照的平均光密度值 + 3个标准差）。当通过蛋白质印迹法检测时，阳性血清也能识别pADPRP蛋白。此外，一些高阳性血清进行免疫沉淀后，酶活性受到抑制。
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