Department of Physics, University of Osnabrück, Barbarastr. 7, 49069 Osnabrück, Germany.
J Biophotonics. 2011 Jun;4(6):453-63. doi: 10.1002/jbio.201000116. Epub 2011 Feb 7.
The design and synthesis of Raman reporter molecules comprising olefin or alkyne moieties with strong and characteristic vibrational Raman bands is presented. Chemisorption onto the surface of colloidal Au/Ag shells yields a self-assembled monolayer. Hydrophilic stabilization of such SERS labels can be achieved by short terminal ethylene glycol units attached to the Raman reporter. Encapsulation by silica with subsequent functionalization of the glass surface allows the conjugation to biomolecules such as antibodies. We demonstrate the use of SERS-labeled antibodies for tissue imaging of the tumor suppressor p63 in prostate biopsies.
介绍了包含烯烃或炔烃部分的喇曼报告分子的设计和合成，这些部分具有强而特征的振动喇曼带。化学吸附到胶体 Au/Ag 壳的表面上产生自组装单层。通过附着在喇曼报告分子上的短末端乙二醇单元可以实现这种 SERS 标记物的亲水稳定化。用硅石封装，然后对玻璃表面进行功能化，允许与生物分子（如抗体）进行缀合。我们展示了使用 SERS 标记的抗体进行前列腺活检中肿瘤抑制因子 p63 的组织成像。
