Ribeiro J, Ballet F, Cynober L, Coudray-Lucas C, Baudrimont M, Legendre C, Delelo R, Arvieux C, Nordlinger B
INSERM, Unité 181, Hôpital Saint-Antoine, Paris.
Chirurgie. 1990;116(8-9):756-61.
The aim of this work was to assess the effect of intrasplenic liver cell transplantation (ILCT) on hepatic insufficiency induced by a terminolateral portocaval shunt (PCS) in rats. Thirty syngenic Wistar Furth rats were divided up into three groups: (a) rats with PCS (n = 10); (b) rats with PCS then ILCT of 10(7) liver cells isolated from the livers of syngenic rats (n = 10); (c) operated control rats (n = 10). Double-blind behavior tests were carried out two weeks, two months and six months after surgery. The spontaneous motor activity and the exploring activity of each rat were studied in automated cages fitted with infrared diodes. Each interruption of the infrared beam was automatically recorded by a computer and converted into an activity score (number/hour). The spontaneous motor activity and the exploring activity were poor in the rats with PCS. The ILCT significantly increased the spontaneous motor activity and the exploring activity 2 months and 3 weeks after transplantation, respectively. Three months after transplantation, the spontaneous motor activity and the exploring activity in the PCS/ILCT group were not significantly different from those of the control rats. This study shows that ILCT can correct the neurological signs of hepatic encephalopathy in an experimental model of chronic hepatic insufficiency, and suggests that ILCT may produce therapeutic benefits in chronic hepatic insufficiency.
本研究旨在评估脾内肝细胞移植（ILCT）对大鼠端侧门腔静脉分流术（PCS）所致肝功能不全的影响。将30只同基因Wistar Furth大鼠分为三组：（a）行PCS的大鼠（n = 10）；（b）行PCS后再移植从同基因大鼠肝脏分离的10⁷个肝细胞的大鼠（n = 10）；（c）手术对照大鼠（n = 10）。在术后两周、两个月和六个月进行双盲行为测试。在装有红外二极管的自动笼中研究每只大鼠的自发运动活动和探索活动。红外光束的每次中断都由计算机自动记录并转换为活动评分（次数/小时）。行PCS的大鼠自发运动活动和探索活动较差。ILCT分别在移植后2个月和3周显著增加了自发运动活动和探索活动。移植后三个月，PCS/ILCT组的自发运动活动和探索活动与对照大鼠无显著差异。本研究表明，ILCT可纠正慢性肝功能不全实验模型中肝性脑病的神经学体征，并提示ILCT可能对慢性肝功能不全产生治疗益处。
