Molecular Imaging Program at Stanford, Department of Radiology, Stanford, CA 94305, USA.
Small. 2011 Mar 7;7(5):625-33. doi: 10.1002/smll.201002291. Epub 2011 Feb 8.
The affibody functionalization of fluorescent surface-enhanced Raman scattering gold-silica nanoparticles as multimodal contrast agents for molecular imaging specific to epidermal growth factor receptor (EGFR) is reported. This nanoparticle bioconjugate reports EGFR-positive A431 tumors with a signal nearly 35-fold higher than EGFR-negative MDA-435S tumors. The low-level EGFR expression in adjacent healthy tissue is 7-fold lower than in the positive tumors. Validation via competitive inhibition reduces the signal by a factor of six, and independent measurement of EGFR via flow cytometry correlates at R(2) = 0.92.
本文报道了荧光表面增强拉曼散射金硅纳米粒子的亲和体功能化,将其作为针对表皮生长因子受体 (EGFR) 的分子成像的多模式对比剂。这种纳米粒子生物缀合物报告了 EGFR 阳性的 A431 肿瘤,其信号比 EGFR 阴性的 MDA-435S 肿瘤高近 35 倍。相邻健康组织中低水平的 EGFR 表达比阳性肿瘤低 7 倍。通过竞争性抑制进行验证,信号降低了六倍,通过流式细胞术独立测量 EGFR 的相关性为 R(2) = 0.92。