Zhou Bi-ying, Chen Ya-tang, Li Wen-gui, Yang Mei
Institute of Infectious and Parasitic Diseases, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2010 Sep;41(5):764-6.
To dynamically observe changes of subsets of splenocytes in mice immunized with recombinant Bifidobacteria bifidum (Bb)-Eg95-EgA31 vaccine of Echinococcus granulosus (Eg).
BALB/c mice were vaccinated by 5 x 10(8) colony forming unit (CFU) orally and 5 x 10(5) CFU intranasally respectively. Mice were killed on week 0, 2, 4, 6, 8, 10, 12, 14, 16, 18 and 20 after immunization respectively, and spleens were separated for cell preparation. CD4+ and CD8+ T cells were determined by flow cytometry (FCM), with MRS as control.
In the oral immunization group, CD4+ cells showed a significant increase during the 4th-10th week after vaccination, and reached the highest level on the 6th week, whereas no obvious changes in CD8+ cells numbers were observed. In the intranasal immunization group, CD4+ cells showed an obvious increase during the 4th-8th week after vaccination, and reached the highest level on the 6th week, CD8+ subsets had no obvious changes.
CD4+ T cell cells may play a key role in immune response in mice immunized with the recombinant Bb-Eg95-EgA31 vaccine of Echinococcus granulosus.
动态观察用重组双歧双歧杆菌（Bb）-细粒棘球绦虫（Eg）Eg95-EgA31疫苗免疫小鼠后脾细胞亚群的变化。
分别经口给予BALB/c小鼠5×10⁸ 菌落形成单位（CFU）、经鼻给予5×10⁵ CFU进行疫苗接种。分别在免疫后第0、2、4、6、8、10、12、14、16、18和20周处死小鼠，分离脾脏进行细胞制备。采用流式细胞术（FCM）检测CD4⁺和CD8⁺ T细胞，以MRS作为对照。
经口免疫组，接种疫苗后第4至10周CD4⁺细胞显著增加，第6周达到最高水平，而CD8⁺细胞数量未观察到明显变化。经鼻免疫组，接种疫苗后第4至8周CD4⁺细胞明显增加，第6周达到最高水平，CD8⁺亚群无明显变化。
CD4⁺ T细胞可能在用重组Bb-Eg95-EgA31细粒棘球绦虫疫苗免疫的小鼠免疫反应中起关键作用。
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