[重组卡介苗- Eg95疫苗诱导小鼠抗细粒棘球绦虫的脾淋巴细胞因子变化]

[Change of splenocyte lymphokines in mice induced by recombinant BCG-Eg95 vaccine against Echinococcus granulosus].

作者信息

Li Wen-Gui, Wang Hong, Zhu You-Ming

机构信息

Institute of Infectious and Parasitic Diseases, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, China.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2007 Apr 30;25(2):109-13.

PMID:17633820

Abstract

OBJECTIVE

To investigate the reduction of hydatid cyst weight and change of splenocyte lymphokines in mice immunized with recombinant BCG-Eg95 vaccine of Echinococcus granulosus(Eg).

METHODS

BALB/C mice were subcutaneously, intranasally, orally and intramuscularly vaccinated respectively, with BCG and PBS served as controls. The mice were challenged with Eg protoscolices 8 weeks after vaccination and sacrificed in 18 weeks after infection. The weight of hydatid cyst was measured and reduction of the weight was obtained, spleens were used to separate splenocytes which were cultured under stimulation with EgAg or ConA. The supernatant was collected to measure the level of IL-2, IFN-gamma, TNF-alpha and IL-4 by ELISA.

RESULTS

The hydatid cyst weight reduced by 45.77%, 18.20%, 88.05% and 92.46% respectively in the 4 immunization groups. In comparison with PBS control, the level of IL-2, IFN-gamma and TNF-alpha was (30.0+/-0) pg/ml, (65.0+/-0) pg/ml and (425.0+/-10.7) pg/ml respectively in the intramuscular group with a significant increase, but that of IL-4 decreased, with a value of (10.0+/-0) pg/ml.

CONCLUSION

The recombinant BCG-Eg95 vaccination induces Thl response in mice against challenge infection.

摘要

目的

研究用细粒棘球绦虫重组卡介苗- Eg95疫苗免疫小鼠后，其体内棘球蚴囊肿重量的减轻情况及脾细胞淋巴因子的变化。

方法

将BALB/C小鼠分别经皮下、鼻内、口服和肌肉注射接种疫苗，以卡介苗和PBS作为对照。接种疫苗8周后用细粒棘球绦虫原头节攻击小鼠，并在感染后18周处死。测量棘球蚴囊肿的重量并计算重量减轻率，取脾脏分离脾细胞，在细粒棘球绦虫抗原（EgAg）或刀豆蛋白A（ConA）刺激下培养。收集上清液，用酶联免疫吸附测定法（ELISA）检测白细胞介素-2（IL-2）、γ干扰素（IFN-γ）、肿瘤坏死因子-α（TNF-α）和白细胞介素-4（IL-4）的水平。

结果

4个免疫组的棘球蚴囊肿重量分别减轻了45.77%、18.20%、88.05%和92.46%。与PBS对照组相比，肌肉注射组的IL-2、IFN-γ和TNF-α水平分别为（30.0±0）pg/ml、（65.0±0）pg/ml和（425.0±10.7）pg/ml，显著升高，而IL-4水平降低，为（10.0±0）pg/ml。