Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, People's Republic of China.
Gene. 2011 Jun 15;479(1-2):1-9. doi: 10.1016/j.gene.2011.01.015. Epub 2011 Feb 16.
The lytic and lysogenic life cycle switch of bacteriophages plays very important roles in virus-host interactions. However, the lysogeny of thermophilic bacteriophage infecting thermophile at high temperatures has not been addressed. In this study, two lysogeny-related genes encoding the CI protein and recombinase of GVE2, a thermophilic bacteriophage obtained from a deep-sea hydrothermal vent, were characterized. Temporal analyses showed that the two genes were expressed at early stages of GVE2 infection. Based on chromatin immunoprecipitation (ChIP) assay and electrophoretic mobility shift assay (EMSA), the GVE2 CI protein was bound with only one DNA fragment located at 24264-24036 bp in the GVE2 genome. This location might be the original transcription site and the lysis-lysogeny switch site, which was very different from mesophilic bacteriophages. The GVE2 CI and recombinase proteins could function only at high temperatures. Therefore our study improved our understanding of the lysogeny process of bacteriophages at high temperatures.
噬菌体的裂解和溶源生活周期转换在病毒-宿主相互作用中起着非常重要的作用。然而，在高温下感染嗜热菌的嗜热噬菌体的溶源现象尚未得到解决。在这项研究中，我们对来自深海热液喷口的一种嗜热噬菌体 GVE2 的两个溶源相关基因（编码 CI 蛋白和重组酶）进行了表征。时程分析表明，这两个基因在 GVE2 感染的早期阶段表达。基于染色质免疫沉淀（ChIP）分析和电泳迁移率变动分析（EMSA），GVE2 的 CI 蛋白仅与 GVE2 基因组中 24264-24036bp 处的一个 DNA 片段结合。该位置可能是原始转录位点和裂解-溶源转换位点，这与中温噬菌体有很大的不同。GVE2 的 CI 和重组酶蛋白只能在高温下发挥作用。因此，我们的研究提高了我们对高温下噬菌体溶源过程的理解。
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