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嗜热栖热放线菌嗜热噬菌体的特性分析。

Characterization of a thermophilic bacteriophage of Geobacillus kaustophilus.

作者信息

Marks Timothy J, Hamilton Paul T

机构信息

Department of Pharmaceutical Sciences, Campbell University, PO Box 1090, Buies Creek, NC, 27506, USA.

出版信息

Arch Virol. 2014 Oct;159(10):2771-5. doi: 10.1007/s00705-014-2101-8. Epub 2014 May 6.

Abstract

GBK2 is a bacteriophage, isolated from a backyard compost pile, that infects the thermophile Geobacillus kaustophilus. GBK2 has a circularly permuted genome of 39,078 bp with a G+C content of 43 %. Annotation of the genome reveals 62 putative open reading frames (ORFs), 25 of which (40.3 %) show homology to known proteins and 37 of which (59.7 %) are proteins with unknown functions. Twelve of the identified ORFs had the greatest homology to genes from the phage SPP1, a phage that infects the mesophile Bacillus subtilis. The overall genomic arrangement of GBK2 is similar to that of SPP1, with the majority of GBK2 SPP1-like genes coding for proteins involved in DNA replication and metabolism.

摘要

GBK2是一种从后院堆肥中分离出来的噬菌体,它感染嗜热栖热放线菌。GBK2有一个39078 bp的环状排列基因组,G+C含量为43%。基因组注释显示有62个推定的开放阅读框(ORF),其中25个(40.3%)与已知蛋白质具有同源性,37个(59.7%)是功能未知的蛋白质。已鉴定的12个ORF与噬菌体SPP1的基因具有最高同源性,SPP1是一种感染嗜温枯草芽孢杆菌的噬菌体。GBK2的整体基因组排列与SPP1相似,GBK2中大多数类似SPP1的基因编码参与DNA复制和代谢的蛋白质。

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