Department of Pathology, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
Oncol Rep. 2011 Apr;25(4):929-35. doi: 10.3892/or.2011.1176. Epub 2011 Feb 9.
There is strong evidence that the angiopoietin family is involved in the regulation of tumour progression. Recently, it has been reported that angiopoietin-like 4 (ANGPTL4) expression in cancer cells promotes the metastatic process by increasing vascular permeability. The present study was conducted to examine ANGPTL4 expression and its association with clinicopathological factors and prognosis in human colorectal cancers. We examined 144 cases of surgically-resected human colorectal adenocarcinomas by immunohistochemistry, RT-PCR and Western blot analysis. Also, overall survival was investigated. Among 144 cases of adenocarcinoma, 95 cases (66.0%) showed positive staining in the cytoplasm of the carcinoma cells for ANGPTL4. Histologically, well, moderately, poorly differentiated adenocarcinoma or mucinous carcinoma showed 55.2, 79.3, 61.5 or 44.4% expression of ANGPTL4, respectively. The expression of ANGPTL4 was correlated with the depth of tumour invasion (p<0.0005), Vienna classification (category 3-5)(p<0.00005), venous invasion (p<0.0005) and Duke's classification (p<0.005). However, ANGPTL4 expression was not correlated with overall survival. However, all patients (100%) with distant metastasis showed immunopositivity for ANGPTL4. The mRNA and the protein expression of ANGPTL4 were shown in four resected samples and cultured cell lines by RT-PCR or western blot analysis. These findings suggest that ANGPTL4 is one of the factors involved in the progression of human colorectal cancer, especially venous invasion and distant metastasis.
有强有力的证据表明，血管生成素家族参与了肿瘤的进展调控。最近有报道称，癌细胞中的血管生成素样蛋白 4（ANGPTL4）表达通过增加血管通透性促进转移过程。本研究旨在检测人类结直肠癌中 ANGPTL4 的表达及其与临床病理因素和预后的关系。我们通过免疫组织化学、RT-PCR 和 Western blot 分析检测了 144 例手术切除的人结直肠腺癌病例。还调查了总生存率。在 144 例腺癌病例中，95 例（66.0%）在癌细胞的细胞质中 ANGPTL4 呈阳性染色。组织学上，高分化、中分化、低分化或黏液腺癌的 ANGPTL4 表达分别为 55.2%、79.3%、61.5%或 44.4%。ANGPTL4 的表达与肿瘤浸润深度（p<0.0005）、维也纳分类（类别 3-5）（p<0.00005）、静脉侵犯（p<0.0005）和 Duke 分类（p<0.005）相关。然而，ANGPTL4 的表达与总生存率无关。然而，所有远处转移的患者（100%）均对 ANGPTL4 呈免疫阳性。通过 RT-PCR 或 Western blot 分析在四个切除样本和培养细胞系中显示了 ANGPTL4 的 mRNA 和蛋白表达。这些发现表明 ANGPTL4 是参与人类结直肠癌进展的因素之一，尤其是静脉侵犯和远处转移。
