Division of Environmental and Water Resources Engineering, School of Civil and Environmental Engineering, Nanyang Technological University, Singapore.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2011;46(3):314-22. doi: 10.1080/10934529.2011.539117.
In this study, biofilms were developed in three airlift reactors to treat wastewaters with ampicillin (AMP) of 0, 4 and 8 mg L(-1), respectively. During 60 days of operation, AMP was not found to inhibit the biofilm growth. Denaturing gradient gel electrophoresis (DGGE) and 16S rRNA gene sequencing were used to characterize the bacterial community of these biofilms. It was found that the community diversity was lowered, whereas the community stability was enhanced in the biofilm supplemented with AMP as compared to the biofilm free of AMP. Community members were particularly examined in the biofilms developed with 8 mg L(-1) AMP at different stages. Phylogenetic classification revealed that all the identified bacteria fell into four divisions: β-Proteobacteria, α-Proteobacteria, γ-Proteobacteria and Bacteroides. The dominant genus was Acidovorax sp. with an abundance of about 35%. Further analyses on the identification results showed that the quantitative change of AMP-degrading bacteria in the biofilms developed with AMP was positively related to the AMP biodegradation performance.
在这项研究中，采用三种气升式反应器来处理分别含有 0、4 和 8 mg/L 氨苄青霉素（AMP）的废水，以形成生物膜。在 60 天的运行过程中，并未发现 AMP 抑制生物膜的生长。采用变性梯度凝胶电泳（DGGE）和 16S rRNA 基因测序技术对这些生物膜中的细菌群落进行了表征。与不含 AMP 的生物膜相比，添加 AMP 的生物膜的群落多样性降低，而群落稳定性增强。在含有 8 mg/L AMP 的生物膜中，特别对不同阶段的生物膜进行了群落成员的检查。系统发育分类表明，所有鉴定的细菌均属于四个分类：β-变形菌门、α-变形菌门、γ-变形菌门和拟杆菌门。优势属为食酸菌属（Acidovorax sp.），丰度约为 35%。对鉴定结果的进一步分析表明，生物膜中 AMP 降解菌的数量变化与 AMP 的生物降解性能呈正相关。
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