Gennari Oriella, Montesano Domenico, Salzano Antonio, Albrizio Stefania, Grumetto Lucia
Dipartimento di Chimica Farmaceutica e Tossicologica, Università degli Studi di Napoli Federico II, Via D. Montesano, 49, I-80131, Naples, Italy.
Biomed Chromatogr. 2011 Dec;25(12):1315-8. doi: 10.1002/bmc.1602. Epub 2011 Feb 10.
A method for the determination of dimethyl fumarate (DMF) in desiccant and antimould sachets, employed for protecting consumer products from humidity and mould, has been developed. The method is based on a solid-liquid extraction followed by HPLC-UV analysis. The method was validated with respect to recovery, linearity, limits of detection and quantitation and precision. The recovery was 98%. The correlation coefficient value (r) was equal to 0.94. Both intra- and inter-day precisions were studied at several concentration levels, being satisfactory in all cases (RSD < 5). Limits of detection and quantification values were in the low microgram per gram level, thus allowing the determination of DMF at concentrations below the limit established (0.1 mg/kg) by the recent EU Directive (Decision 2009/251/EC). The proposed procedure was applied for the determination of the target compound in 41 desiccant and antimould samples. DMF was detected in 39.0% of samples and its content in many samples exceeded the legal limits. The results of our analysis highlight the high risk of exposure to this powerful allergic sensitizer for consumers.
已开发出一种用于测定干燥剂和防霉香囊中富马酸二甲酯（DMF）的方法，这些干燥剂和防霉香囊用于保护消费品免受潮湿和霉菌侵害。该方法基于固液萃取，随后进行HPLC-UV分析。该方法在回收率、线性、检测限和定量限以及精密度方面进行了验证。回收率为98%。相关系数值（r）等于0.94。在几个浓度水平上研究了日内和日间精密度，所有情况下均令人满意（RSD < 5）。检测限和定量限的值处于每克低微克水平，因此能够测定低于欧盟近期指令（2009/251/EC号决定）规定限值（0.1 mg/kg）的DMF浓度。所提出的方法应用于41个干燥剂和防霉样品中目标化合物的测定。在39.0%的样品中检测到了DMF，许多样品中的含量超过了法定限值。我们的分析结果凸显了消费者接触这种强效过敏致敏剂的高风险。
