Bermudez Dieldrich S, Skotko Jeremy P, Ohta Yasuhiko, Boggs Ashley S P, Iguchi Taisen, Guillette Louis J
Department of Biology, 220 Bartram Hall, University of Florida, Gainesville, FL 32611, USA.
J Morphol. 2011 Jun;272(6):698-703. doi: 10.1002/jmor.10936. Epub 2011 Feb 9.
The expression of estrogen receptors, ESR1 (ERα) and ESR2 (ERβ), and androgen receptors (AR) in the thyroid gland has been reported in few vertebrate species other than a few mammals. This study reports the presence of sex steroid hormone receptors and thyroid receptors (ERα, ERβ, AR, TRα, and TRβ) in the thyroid gland of the American alligator at several life stages. It provides a semiquantification and distribution of ERα in the thyroid follicle cells using an immunohistochemical approach as well as reports quantitative differences in mRNA expression of ERα, ERβ, TRα, TRβ, and AR in the same tissue using quantitative real time-PCR (Q-PCR) with primers designed specifically for alligators. The thyroid tissue of the American alligator expresses ERα, ERβ, and AR at all of the life stages examined here although no statistically significant differences were observed between male and female in thyroid mRNA expression for any of the genes analyzed. No sexual dimorphism was observed in ERα immunostaining. No statistical analysis across life stages were performed due to confounding factor of season.
除少数哺乳动物外，在其他脊椎动物物种中，甲状腺中雌激素受体ESR1（ERα）、ESR2（ERβ）和雄激素受体（AR）的表达鲜有报道。本研究报告了美国短吻鳄在几个生命阶段甲状腺中存在性类固醇激素受体和甲状腺受体（ERα、ERβ、AR、TRα和TRβ）。本研究使用免疫组织化学方法对甲状腺滤泡细胞中的ERα进行了半定量和分布分析，并使用专门为短吻鳄设计的引物，通过定量实时PCR（Q-PCR）报告了同一组织中ERα、ERβ、TRα、TRβ和AR的mRNA表达量的差异。美国短吻鳄的甲状腺组织在本研究的所有生命阶段均表达ERα、ERβ和AR，不过在所分析的任何基因的甲状腺mRNA表达中，未观察到雄性和雌性之间存在统计学显著差异。在ERα免疫染色中未观察到性别二态性。由于季节的混杂因素，未对不同生命阶段进行统计分析。
