极低频电磁场（EMF）对培养细胞中磷脂酶活性的影响。
Effect of Extremely Low Frequency Electromagnetic Fields (EMF) on Phospholipase Activity in the Cultured Cells.
机构信息
College of Pharmacy, Chung-Ang University, Seoul 156-756, Korea.
出版信息
Korean J Physiol Pharmacol. 2010 Dec;14(6):427-33. doi: 10.4196/kjpp.2010.14.6.427. Epub 2010 Dec 31.
Abstract
This study was conducted to investigate the effects of extremely low frequency electromagnetic fields (EMF) on signal pathway in plasma membrane of cultured cells (RAW 264.7 cells and RBL 2H3 cells), by measuring the activity of phospholipase A(2) (PLA(2)), phospholipase C (PLC) and phospholipase D (PLD). The cells were exposed to the EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h. The basal and 0.5 µM melittin-induced arachidonic acid release was not affected by EMF in both cells. In cell-free PLA(2) assay, we failed to observe the change of cPLA(2) and sPLA(2) activity. Also both PLC and PLD activities did not show any change in the two cell lines exposed to EMF. This study suggests that the exposure condition of EMF (60 Hz, 0.1 or 1 mT) which is 2.4 fold higher than the limit of occupational exposure does not induce phospholipases-associated signal pathway in RAW 264.7 cells and RBL 2H3 cells.
摘要
本研究旨在通过测量磷脂酶 A2（PLA2）、磷脂酶 C（PLC）和磷脂酶 D（PLD）的活性，研究极低频电磁场（EMF）对培养细胞（RAW 264.7 细胞和 RBL 2H3 细胞）质膜信号通路的影响。细胞暴露于 EMF（60 Hz，0.1 或 1 mT）4 或 16 小时。在两种细胞中，EMF 对基础和 0.5 μM 蜂毒素诱导的花生四烯酸释放没有影响。在细胞游离 PLA2 测定中，我们未能观察到 cPLA2 和 sPLA2 活性的变化。此外，暴露于 EMF 的两种细胞系中，PLC 和 PLD 活性均未发生变化。本研究表明，暴露于 EMF（60 Hz，0.1 或 1 mT）的条件（比职业暴露限值高 2.4 倍）不会诱导 RAW 264.7 细胞和 RBL 2H3 细胞中与磷脂酶相关的信号通路。
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