细胞色素C及相关金属蛋白中的快速电子转移过程。
Fast electron transfer processes in cytochrome C and related metalloproteins.
作者信息
Simic M G, Taub I A
出版信息
Biophys J. 1978 Oct;24(1):285-94. doi: 10.1016/S0006-3495(78)85377-6.
Various free radicals formed on pulse radiolysis of aqueous solutions have been used to investigate the mechanisms of reduction of cytochrome(III) c by inter- and intramolecular electron transfer. The rapid formation of free radicals (t less than 1 mus) and their high reactivity with cytochrome (k approximately 10(8)(-5) x 10(10)M(-1)s(-1)) make such studies feasible. Reduction of cytochrome by free radicls is monitored by optical methods. Fast optical changes in the 1(-500)-mus region correspond to reduction of the iron center; whereas the slower changes in the 10(-500)-ms region are attributed to postreduction conformational changes. It has been concluded that the reduction path is mediated through the crevice and that no reduction intermediates are being formed.
水溶液脉冲辐解产生的各种自由基已被用于研究分子间和分子内电子转移还原细胞色素（III）c的机制。自由基的快速形成（t小于1微秒）及其与细胞色素的高反应性（k约为10⁸（-5）×10¹⁰M⁻¹s⁻¹）使此类研究成为可能。通过光学方法监测自由基对细胞色素的还原。1（-500）-微秒区域的快速光学变化对应于铁中心的还原；而10（-500）-毫秒区域较慢的变化归因于还原后的构象变化。已经得出结论，还原途径是通过裂隙介导的，并且没有形成还原中间体。
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