Fedotov V P, Komolov I S, Pluzhnikova G N
Biull Eksp Biol Med. 1978 Oct;86(10):491-3.
Some peculiarities of labeled growth hormone (GH) and prolactin (PL) secretion in the 5-day monolayer culture of the rat adenohypophysis was studied. The hormones from the culture medium were obtained by electrophoresis on polyacrylamide gel. Natrium-dibutyril of cyclic adenosine-3',5'-monophosphate and theophylline, stimulated the GH and PL secretion. Thyrotropin-releasing hormone (TRH) increased the incorporation of 14C-1-leucine into the cell protein, stimulated PL secretion, but did not act on the GH release. Somatostatin completely abolished the GH secretion mediated by theophyllin, but not that of PL. Some peculiarities in the formation of labeled GH and PL pool in the cells and secretion of these hormones into the culture medium are discussed.
研究了大鼠腺垂体5天单层培养中标记生长激素（GH）和催乳素（PL）分泌的一些特性。通过聚丙烯酰胺凝胶电泳从培养基中获取激素。环磷酸腺苷3'，5'-单磷酸二丁酯钠和茶碱刺激了GH和PL的分泌。促甲状腺激素释放激素（TRH）增加了14C-1-亮氨酸掺入细胞蛋白的量，刺激了PL分泌，但对GH释放没有作用。生长抑素完全消除了茶碱介导的GH分泌，但没有消除PL的分泌。讨论了细胞中标记GH和PL池的形成以及这些激素向培养基分泌的一些特性。
