二丁酰环磷腺苷（dbcAMP）对溶血卵磷脂诱导的犊牛和成年牛红细胞溶血的影响。

The effect of dbcAMP on the lysolecithin induced hemolysis of calf and adult cattle erythrocytes.

作者信息

Imre S

出版信息

Blut. 1978 Nov 13;37(5):257-64. doi: 10.1007/BF01539661.

Abstract

The calf erythrocytes have an increased sensitivity against lysolecithin as compared to their adult counterparts. 10(-3)M dbcAMP increases the hemolysis induced by 5 microgram of lysolecithin in 0.15 M NaCl containing 10 mM phosphate buffer (pH 7.4). By increasing the level of phosphate buffer (75 mM) in the incubation mixture, 10(-3)M dbcAMP decreases the hemolysis induced by 5 microgram of lysolecithin. These data suggest a dual effect exerted by dcbAMP: the relatively labilizing or stabilizing effect prevails as a function of exogenous inorganic phosphate level. 10(-6)M dcbAMP also has a relative protective effect against lysolecithin. The combined addition of cAMP (10(-3)) and theophyllin (10(-4)M) does not stabilize the membrane. By increasing the level of lysolecithin to 20 microgram/ml the stabilizing effect of dcbAMP disappears. DbcAMP (10(-3)) as well as cAMP (10(-3)M) and theophyllin (10(-4)M) have a minimum increasing effect on hemolysis in the absence of lysolecithin, too.

摘要

与成年小牛的红细胞相比，小牛的红细胞对溶血卵磷脂的敏感性更高。10⁻³M双丁酰环磷腺苷（dbcAMP）会增加在含有10 mM磷酸盐缓冲液（pH 7.4）的0.15 M氯化钠中5微克溶血卵磷脂诱导的溶血作用。通过提高孵育混合物中磷酸盐缓冲液的浓度（75 mM），10⁻³M dbcAMP会降低5微克溶血卵磷脂诱导的溶血作用。这些数据表明双丁酰环磷腺苷（dcbAMP）具有双重作用：相对不稳定或稳定的作用根据外源无机磷酸盐水平占主导。10⁻⁶M dcbAMP对溶血卵磷脂也有相对的保护作用。联合添加环磷腺苷（cAMP，10⁻³）和茶碱（10⁻⁴M）不会使细胞膜稳定。通过将溶血卵磷脂的浓度提高到20微克/毫升，dcbAMP的稳定作用消失。在没有溶血卵磷脂的情况下，双丁酰环磷腺苷（10⁻³）以及环磷腺苷（10⁻³M）和茶碱（10⁻⁴M）对溶血的增加作用也最小。