Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia.
Talanta. 2011 Mar 15;84(1):127-32. doi: 10.1016/j.talanta.2010.12.025. Epub 2010 Dec 25.
Whole grain cereals are an important source of bioavailable lignans, the group of compounds with potential anti-cancerogenic, antioxidant, anti-proliferative, pro-apoptotic, and antiangiogenic properties. The aim of this work was to develop a sensitive method for determination of wheat bran lignans. The analysis of lignans secoisolariciresinol, hydroxymatairesinol, lariciresinol, matairesinol, pinoresinol, syringaresinol is based on derivatization with pentafluoropropionic anhydride (PFPA) and gas chromatography-electron capture detection (GC-ECD), using styrene glycol as internal standard. To our knowledge, this is the first time that EC detection has been used for lignan analysis. The results show that the technique is reproducible and sensitive enough for detecting lignans in wheat at parts-per-billion (ppb) levels, except for hydroxymatairesinol. The method developed showed good recovery (85-105%) and precision (4-20%) for five types of lignans and thus represents a simpler and more affordable alternative to state-of-the-art wheat lignan liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis.
全谷物是生物可利用木脂素的重要来源，木脂素是一组具有潜在抗癌、抗氧化、抗增殖、促凋亡和抗血管生成特性的化合物。本工作旨在开发一种测定麦麸木脂素的灵敏方法。木脂素开环异落叶松脂素、羟落叶松脂素、落叶松脂素、马松脂素、松脂素和丁香树脂醇的分析基于与五氟丙酸酐（PFPA）的衍生化作用，并使用苯乙烯二醇作为内标，采用气相色谱-电子捕获检测（GC-ECD）。据我们所知，这是首次将 EC 检测用于木脂素分析。结果表明，该技术可重现且足够灵敏，可在十亿分之几（ppb）的水平检测小麦中的木脂素，除了羟落叶松脂素。所开发的方法对 5 种木脂素的回收率（85-105%）和精密度（4-20%）均较好，因此代表了一种比最先进的小麦木脂素液相色谱串联质谱（LC-MS/MS）分析更简单、更经济的替代方法。
