Australian Animal Health Laboratories, CSIRO Livestock Industries, Geelong, Victoria, Australia.
Viral Immunol. 2011 Feb;24(1):3-9. doi: 10.1089/vim.2010.0064.
Chicken anemia virus (CAV) is an economically important virus affecting the chicken meat and egg industry. CAV is characterized by anemia, lymphoid depletion, and immunosuppression. Microarrays were used to investigate the response of MDCC-MSB1 cells (MSB1) to infection with CAV at 24 and 48 h post-infection (hpi). The major genes responding to CAV infection include genes involved in inflammation, apoptosis, and antiviral activity. Several cytokines were differentially regulated at either 24 or 48 hpi, including interleukin 2 (IL-2), interleukin receptors IL-1R, IL-22R, IL-18R, and IL-7R, and interferon-α (IFN-α). While there were many genes differentially regulated in this experiment, only two genes were common to both time points, suggesting a dramatic change in gene expression over the two time points studied. The present study is the first microarray experiment to investigate CAV, and we identified a number of key pathways involved in viral infection. Overall, there were more genes upregulated at 24 hpi than at 48 hpi, including genes involved in cytokine signaling, apoptosis, and antiviral activity. The two time points were vastly different in their gene expression patterns, in that at 24 hpi there were many genes involved in the response to infection, whereas at 48 hpi there were many genes associated with apoptosis and immunosuppression.
鸡贫血病毒（CAV）是一种影响鸡肉和鸡蛋产业的具有经济重要性的病毒。CAV 的特征是贫血、淋巴组织耗竭和免疫抑制。本研究使用微阵列技术在感染后 24 和 48 小时（hpi）调查了 MDCC-MSB1 细胞（MSB1）对 CAV 感染的反应。对 CAV 感染有反应的主要基因包括参与炎症、细胞凋亡和抗病毒活性的基因。几种细胞因子在 24 或 48 hpi 时差异调节，包括白细胞介素 2（IL-2）、白细胞介素受体 IL-1R、IL-22R、IL-18R 和 IL-7R，以及干扰素-α（IFN-α）。虽然该实验中有许多基因差异调节，但只有两个基因在两个时间点都有共同之处，这表明在研究的两个时间点上基因表达发生了巨大变化。本研究是首次对 CAV 进行微阵列实验，鉴定了参与病毒感染的多个关键途径。总的来说，在 24 hpi 时上调的基因比在 48 hpi 时更多，包括细胞因子信号、细胞凋亡和抗病毒活性相关基因。这两个时间点在基因表达模式上有很大的不同，在 24 hpi 时，有许多基因参与感染反应，而在 48 hpi 时，有许多基因与细胞凋亡和免疫抑制有关。
