Morin Louis-Philippe, Mess Jean-Nicholas, Furtado Milton, Garofolo Fabio
Algorithme Pharma Inc., 575 Armand-Frappier, Laval, Quebec, Canada.
Bioanalysis. 2011 Feb;3(3):275-83. doi: 10.4155/bio.10.203.
Louis-Philippe Morin is a senior instrument application specialist at Algorithme Pharma, a CRO located in Laval, Canada. He has been working in the bioanalysis industry for the past 10 years where he became a subject matter expert in analytical instrumentation, especially in the MS field. His responsibilities in his current position are to optimize the workflow of the laboratory and to find new procedures, or approaches, to fix complex analytical problems. Louis-Philippe's expertise acquired over the years has led him to multiple publications regarding instrumentation. LC-MS/MS is the analytical technique of choice for the quantification of drugs in biological fluids. In recent years, MS/MS detection has been impacted by the rapid evolution of bioanalysis industry requirements. The availability of fast chromatographic systems, the demand for wider dynamic ranges and the extensive use of stable isotope-labeled internal standards in bioanalysis has pushed some triple quadrupole detectors to their limits of operation. Consequently, this situation has led to a re-evaluation of the problem of crosstalk as a potential cause of issues in bioanalysis. In this article, the importance of crosstalk verification on the MS/MS instrument will be demonstrated. Additionally, procedures to identify, evaluate and fix possible crosstalk issues during bioanalytical assays on MS/MS instruments are proposed.
路易 - 菲利普·莫林是位于加拿大拉瓦尔的合同研究组织（CRO）Algorithme Pharma的高级仪器应用专家。在过去十年中，他一直从事生物分析行业工作，在此期间他成为了分析仪器领域的专家，尤其是在质谱（MS）领域。他目前职位的职责是优化实验室的工作流程，并寻找新的程序或方法来解决复杂的分析问题。路易 - 菲利普多年来积累的专业知识使他发表了多篇关于仪器方面的文章。液相色谱 - 串联质谱（LC - MS/MS）是生物流体中药物定量分析的首选技术。近年来，串联质谱检测受到生物分析行业需求快速发展的影响。快速色谱系统的可用性、对更宽动态范围的需求以及生物分析中稳定同位素标记内标物的广泛使用，已使一些三重四极杆检测器达到其操作极限。因此，这种情况导致人们重新评估串扰问题，将其视为生物分析中潜在问题的一个原因。在本文中，将展示在串联质谱仪器上进行串扰验证的重要性。此外，还提出了在串联质谱仪器上进行生物分析测定期间识别、评估和解决可能的串扰问题的程序。
