Chen Xiaolan, Lu Yang, Du Shouying, Yao Zongling, Wang Shan, Li Pengyue
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Zhongguo Zhong Yao Za Zhi. 2010 Nov;35(21):2826-8.
To establish a sensitive HPLC method for determining the concentrations of paeonol in rat plasma and to evaluate its pharmacokinetic characteristics.
The paeonol from eortex Moutan was distilled by the way of water-vapor. A single i.v. dose of 4 mg x kg(-1) paeonol injection was given to 5 health rats. Paeonol was separated on a Diamonsil -C18 column with methanol-water (60: 40)as mobile phase. The plasma concentrations of paeonol were determined and its pharmacokinetic parameters were calculated and evaluated by using kinetica 4.0.
The linear range of the method for paeonol was 0.204-20.4 mg x L(-1) and the determination limit was 0.204 mg x L(-1). The main pharmacokinetic parameters, such as AUC, MRT, C(max), Kel, t(1/2kel), after a single dose of paeonol injection were (111.88 +/- 14.44) mg x L(-1) x min(-1), (23.25 +/- 5.86) min, (8.99 +/- 0.84) mg x L(-1), (0.082 +/- 0.015) min(-1) and (8.73 +/- 1.54) min, respectively.
The HPLC method for determining paeonol concentration in plasma is simple, rapid, sensitive and suitable for pharmacokinetic studies.
建立一种灵敏的高效液相色谱法测定大鼠血浆中丹皮酚的浓度，并评价其药代动力学特征。
采用水蒸气蒸馏法从牡丹皮中提取丹皮酚。给5只健康大鼠静脉注射4mg·kg⁻¹丹皮酚注射液。丹皮酚在Diamonsil -C18柱上以甲醇-水（60:40）为流动相进行分离。测定血浆中丹皮酚的浓度，并使用Kinetica 4.0计算和评价其药代动力学参数。
该方法测定丹皮酚的线性范围为0.204～20.4mg·L⁻¹，测定限为0.204mg·L⁻¹。单次注射丹皮酚后，主要药代动力学参数AUC、MRT、C(max)、Kel、t(1/2kel)分别为(111.88±14.44)mg·L⁻¹·min⁻¹、(23.25±5.86)min、(8.99±0.84)mg·L⁻¹、(0.082±0.015)min⁻¹和(8.73±1.54)min。
该高效液相色谱法测定血浆中丹皮酚浓度简便、快速、灵敏，适用于药代动力学研究。
