Xi Shengyan, Zhang Qian, Liu Chaoyang, Xie Hua, Yue Lifeng, Zhao Yufang, Zang Baoxia, Gao Xuemin
Department of Traditional Chinese Medicine of Medical College of Xiamen University, Xiamen 361005, China.
Zhongguo Zhong Yao Za Zhi. 2010 Nov;35(21):2877-81.
To investigate the effects of hydroxy safflor yellow A (HSYA) on the expression of bFGF protein and MMP-9 mRNA or protein of transplantation tumor of gastric adenocarcinoma cell line BGC-823 in nude mice.
The BGC-823 cells were subcutaneously injected into the right anterior armpit of BALB/C nu/nu nude mice, and the animal model of transplantation tumor was established. The experimental groups were treated with HSYA at concentration of 0.056 and 0.028 g x L(-1) and cyclophosphamide at 2 g x L(-1), or with physiologic saline. The tumor inhibitory effect was observed, and the mRNA expression of MMP-9 of transplantation tumor was detected by real time-fluorescent quantitation PCR and the protein expression of MMP-9 and bFGF were detected by enzyme linked immunosorbent assay.
The IR in the group with HSYA at the concentration of 0.028 g x L(-1) is higher than in the group with normal sodium. After treatment with HSYA, the mRNA expression of MMP-9 has significant difference at the concentration of 0.028 g x L(-1) as compared with physiologic saline-treated group (P < 0.05), but the protein expression of MMP-9 and bFGF is obviously less than that in the physiologic saline-treated group (P < 0.05).
The possible mechanism of HSYA in given concentration to antagonize tumor angiogenesis may be related with inhibiting the protein expression of MMP-9 and bFGF or the mRNA expression of MMP-9 in tumor tissue to reduce the degradation of blood vessel basilar membrane, and to restrain the migration of blood vessel and decrease the tumor vascularization.
研究羟基红花黄色素A（HSYA）对人胃腺癌BGC-823细胞株移植瘤裸鼠bFGF蛋白表达及MMP-9 mRNA或蛋白表达的影响。
将BGC-823细胞接种于BALB/C nu/nu裸鼠右腋前线皮下，建立移植瘤动物模型。实验组分别用浓度为0.056、0.028 g·L-1的HSYA及2 g·L-1的环磷酰胺或生理盐水处理。观察肿瘤抑制作用，采用实时荧光定量PCR法检测移植瘤MMP-9的mRNA表达，采用酶联免疫吸附测定法检测MMP-9和bFGF的蛋白表达。
0.028 g·L-1 HSYA组抑瘤率高于生理盐水组。HSYA处理后，0.028 g·L-1浓度组MMP-9的mRNA表达与生理盐水处理组相比差异有统计学意义（P < 0.05），但MMP-9和bFGF的蛋白表达明显低于生理盐水处理组（P < 0.05）。
一定浓度的HSYA拮抗肿瘤血管生成的可能机制可能与抑制肿瘤组织中MMP-9和bFGF的蛋白表达或MMP-9的mRNA表达，减少血管基底膜的降解，抑制血管迁移，降低肿瘤血管化有关。
