清开灵注射液通过嗜碱性粒细胞脱颗粒诱导类过敏反应
[Anaphylactoid reaction induced by Qingkailing injection via basophils cells degranulation].
作者信息
Zheng Xiaoliang, Li Qin, Zhao Yin, Yan Dongmei, Tu Linglan, Zhang Xinyue
机构信息
Institute of Materia Medica, Zhejiang Academy of Medical Sciences, Hangzhou 310013, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Nov;35(21):2904-7.
OBJECTIVE
To observe the effects of Qingkailing injection on RBL-2H3 cell degranulation and histamine release, and discuss the possible mechanism of anaphylactoid reaction induced by Qingkailing injection.
METHOD
RBL-2H3 cells were incubated with Qingkailing injection for 30 min. Then the morphological changes of cells were observed by transmission electron microscopy. Cell degranulation rate was detected by Alcian blue dye assay, Annexin V binding assay and beta-hexosaminidase assay, and cell histamine release rate was detected by ELISA.
RESULT
Different concentration of Qingkailing injection can induce the typical morphological changes in RBL-2H3 cell with degranulation. The rates of degranulation and histamine release in Qingkailing injection treated cells were significantly increased and dose-dependent.
CONCLUSION
RBL-2H3 cell degranulation and histamine release can be induced by single administration of Qingkailing injection, and then induced anaphylactoid reaction, which may be one of the possible mechanisms of serious adverse induced by Qingkailing injection for the first administration in clinic.
目的
观察清开灵注射液对RBL - 2H3细胞脱颗粒及组胺释放的影响，探讨清开灵注射液致类过敏反应的可能机制。
方法
将清开灵注射液与RBL - 2H3细胞孵育30分钟。然后通过透射电子显微镜观察细胞的形态变化。采用阿尔辛蓝染色法、膜联蛋白V结合法和β - 氨基己糖苷酶法检测细胞脱颗粒率，采用酶联免疫吸附测定法检测细胞组胺释放率。
结果
不同浓度的清开灵注射液可诱导RBL - 2H3细胞出现典型的脱颗粒形态变化。清开灵注射液处理的细胞脱颗粒率和组胺释放率显著增加，且呈剂量依赖性。
结论
单次给予清开灵注射液可诱导RBL - 2H3细胞脱颗粒和组胺释放，进而引发类过敏反应，这可能是清开灵注射液临床首次给药引起严重不良反应的可能机制之一。
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