Life and Environmental Science, Shimane University, Shimane, Japan.
J Environ Sci Health B. 2011;46(2):181-90. doi: 10.1080/03601234.2011.539147.
Monochlorophenols were degraded to benzoate via phenol by the initial dechlorination and the subsequent conversion of phenol to benzoate in anaerobic sediment samples of estuarine Lake Shinji under methanogenic conditions. To characterize bacteria that dechlorinate 4-chlorophenol and transform phenol to benzoate, we analyzed the microbial community structure of the enrichment culture with each 4-chlorophenol and phenol by the limiting dilution method with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) of 16S rRNA gene. After serial dilution of the culture, the 4-chlorophenol-dechlorinating culture consisted of two dominant bacteria, one of which was most homologous with Dehalobacter sp. In the enriched culture with phenol, minor band homologous with Cryptanaerobacter phenolicass corresponded to the transformation activity.
在产甲烷条件下，河口湖 Shinji 的厌氧沉积物样品中，一氯酚通过初始脱氯和随后苯酚转化为苯甲酸盐，被降解为苯甲酸盐。为了表征脱氯 4-氯酚和将苯酚转化为苯甲酸盐的细菌，我们通过聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）对每个 4-氯酚和苯酚的限制稀释法，分析了富集培养物的微生物群落结构。16S rRNA 基因。在培养物的连续稀释后，脱氯 4-氯酚的培养物由两种优势细菌组成，其中一种与 Dehalobacter sp. 最同源。在含有苯酚的富集培养物中，与 Cryptanaerobacter phenolicass 同源的少量带与转化活性相对应。
