Department of Chemistry, Faculty of Sciences, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
Electrophoresis. 2011 Mar;32(6-7):764-71. doi: 10.1002/elps.201000607. Epub 2011 Feb 16.
In the present study, a very simple CE method for chiral separation and quantitation of zwitterionic cetirizine (CTZ), as the main metabolite of hydroxyzine (HZ), and HZ has been developed. In addition, the effect of zwitterionic property of CTZ on enantioseparation was investigated. Maltodextrin, a linear polysaccharide, as a chiral selector was used and several parameters affecting the separation such as pH of BGE, concentration of chiral selector and applied voltage were studied. The best BGE conditions for CTZ and HZ enantiomers were optimized as 75 mM sodium phosphate solution at pH of 2.0, containing 5% w/v maltodextrin. Results showed that, compared to HZ, pH of BGE was an effective parameter in enantioseparation of CTZ due to the zwitterionic property of CTZ. The linear range of the method was over 30-1200 ng/mL for all enantiomers of CTZ and HZ. The quantification and detection limits (S/N=3) of all enantiomers were 30 and 10 ng/mL, respectively. The method was used to quantitative enantioseparation of CTZ and HZ in spiked human plasma.
在本研究中，开发了一种非常简单的 CE 方法，用于手性分离和定量测定两性离子西替利嗪（CTZ），作为羟嗪（HZ）的主要代谢物，以及 HZ。此外，还研究了 CTZ 的两性离子性质对对映体分离的影响。使用麦芽糖糊精作为手性选择剂，研究了影响分离的几个参数，如 BGE 的 pH 值、手性选择剂的浓度和施加的电压。优化了 CTZ 和 HZ 对映体的最佳 BGE 条件为 pH 为 2.0 的 75 mM 磷酸钠溶液，其中含有 5%w/v 的麦芽糖糊精。结果表明，与 HZ 相比，由于 CTZ 的两性离子性质，BGE 的 pH 值是对映体分离的有效参数。该方法对所有 CTZ 和 HZ 对映体的线性范围均超过 30-1200 ng/mL。所有对映体的定量和检测限（S/N=3）分别为 30 和 10 ng/mL。该方法用于测定人血浆中 CTZ 和 HZ 的加标对映体分离。
