Richard Dimbleby Department of Cancer Research, Division of Cancer Studies, King's College London, Guy's Medical School Campus, NHH, SE1 1UL, UK.
Chemphyschem. 2011 Feb 25;12(3):442-61. doi: 10.1002/cphc.201000866. Epub 2011 Feb 15.
Herein we discuss how FRET imaging can contribute at various stages to delineate the function of the proteome. Therefore, we briefly describe FRET imaging techniques, the selection of suitable FRET pairs and potential caveats. Furthermore, we discuss state-of-the-art FRET-based screening approaches (underpinned by protein interaction network analysis using computational biology) and preclinical intravital FRET-imaging techniques that can be used for functional validation of candidate hits (nodes and edges) from the network screen, as well as measurement of the efficacy of perturbing these nodes/edges by short hairpin RNA (shRNA) and/or small molecule-based approaches.
在这里，我们讨论了 FRET 成像如何在各个阶段有助于描绘蛋白质组的功能。因此，我们简要描述了 FRET 成像技术、合适的 FRET 对的选择以及潜在的注意事项。此外，我们还讨论了基于 FRET 的最新筛选方法（基于使用计算生物学的蛋白质相互作用网络分析）和临床前活体 FRET 成像技术，这些方法可用于对网络筛选的候选命中（节点和边缘）进行功能验证，以及测量通过短发夹 RNA (shRNA) 和/或基于小分子的方法干扰这些节点/边缘的效果。
