State Key Lab of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, P. R. China.
J Sep Sci. 2011 Mar;34(6):716-23. doi: 10.1002/jssc.201000684. Epub 2011 Feb 15.
C18-functionalized magnetic microspheres synthesized in a three-stage system and characterized by Fourier transform infrared (FTIR) spectroscopy and SEM were applied for clean-up and enrichment of polycyclic aromatic hydrocarbons (PAHs) in soil samples combined with ultrasonication extraction. Magnetic solid-phase extraction (MSPE) parameters, such as elution solvents, amounts of sorbents, enrichment time and organic modifier, were optimized together with ultrasonication time and extraction solvents. Under the optimal conditions, the developed method provided spiked recoveries of 63.2-92.8% with RSDs of less than 6.4% and limits of detection were 0.5-1.0 ng/g. This new method provides several advantages, such as high extraction efficiency, convenient extraction procedure and short analysis times. Finally, the method was successfully applied to the determination of polycyclic aromatic hydrocarbons in soil samples.
C18 功能化磁性微球在三阶段体系中合成，并通过傅里叶变换红外（FTIR）光谱和 SEM 进行表征，用于与超声提取相结合，对土壤样品中的多环芳烃（PAHs）进行净化和富集。磁固相萃取（MSPE）参数，如洗脱溶剂、吸附剂用量、富集时间和有机溶剂，与超声时间和提取溶剂一起进行优化。在最佳条件下，所开发的方法提供了 63.2-92.8%的加标回收率，相对标准偏差（RSD）小于 6.4%，检测限为 0.5-1.0ng/g。该新方法具有提取效率高、提取步骤方便、分析时间短等优点。最后，该方法成功应用于土壤样品中多环芳烃的测定。
