Iablons'ka S V, Rybal'chenko V K
Ukr Biokhim Zh (1999). 2010 May-Jun;82(3):5-17.
Ecto-nucleotidases are enzymes of hydrolase class. They split extracellular nucleoside tri- and diphosphate. In this review a short history of these enzymes investigation, classification, structure, and functional significance of ecto-nucleoside triphosphate diphosphohydrolases (E-NTPDase) has been presented. These enzymes are glycoproteins anchored in membranes. They do not form phosphorylated enzyme's form during catalytic circle, and (by analogy with membrane-bound ATPases) form homooligomeric ensembles. Activity of these enzymes depends on bivalent ions, in particular Ca2+ and Mg2+. E-NTPDases function in the composition of ecto-nucleotidase cascade that contains other nucleotide-hydrolyzing enzymes. They regulate P2-receptors by hydrolyzing its ligand specifically ATP. Both modern information and results of our investigation about influence of different endo- and exogenous factors on activity of these enzymes has been presented.
外核苷酸酶是水解酶类的酶。它们可裂解细胞外核苷三磷酸和二磷酸。在本综述中，介绍了这些酶的研究简史、外核苷三磷酸二磷酸水解酶（E-NTPDase）的分类、结构及功能意义。这些酶是锚定在膜上的糖蛋白。它们在催化循环过程中不会形成磷酸化的酶形式，并且（类似于膜结合ATP酶）形成同寡聚体。这些酶的活性取决于二价离子，特别是Ca2+和Mg2+。E-NTPDase在外核苷酸酶级联反应中发挥作用，该级联反应包含其他核苷酸水解酶。它们通过特异性水解其配体ATP来调节P2受体。本文既介绍了现代信息，也阐述了我们关于不同内源性和外源性因素对这些酶活性影响的研究结果。
