用于检测加工食品中椰奶蛋白的可靠酶联免疫吸附测定法。

Reliable enzyme-linked immunosorbent assay for the determination of coconut milk proteins in processed foods.

机构信息

Institute of Food Research and Product Development (IFRPD), Kasetsart University, 10900, Bangkok, Thailand.

出版信息

J Agric Food Chem. 2011 Mar 23;59(6):2131-6. doi: 10.1021/jf104067v. Epub 2011 Feb 17.

Abstract

This study was designed to develop a novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of coconut milk proteins in processed foods. The developed sandwich ELISA was able to detect coconut milk proteins from various coconut milk products and did not show any cross-reactivity with 41 of 42 kinds of popularly used food ingredients, thus reflecting great specificity for coconut milk proteins. In addition, the established ELISA is highly sensitive and allowed the detection of 0.31 μg/g of coconut milk protein in complex food matrices. This proposed assay could serve as a useful tool for the detection of the presence of hidden coconut milk proteins in processed foods.

摘要

本研究旨在开发一种新颖的夹心酶联免疫吸附测定（ELISA）方法，用于检测和定量加工食品中的椰奶蛋白。所开发的夹心 ELISA 能够检测来自各种椰奶产品的椰奶蛋白，并且与 42 种常用食品成分中的 41 种没有交叉反应，因此对椰奶蛋白具有很好的特异性。此外，建立的 ELISA 具有很高的灵敏度，允许在复杂的食品基质中检测到 0.31μg/g 的椰奶蛋白。该检测方法可作为检测加工食品中隐藏椰奶蛋白的有用工具。