Environmental Engineering Laboratory, Civil Engineering Department, University of Patras, 26500 Patras, Greece.
Colloids Surf B Biointerfaces. 2011 Jun 1;84(2):354-9. doi: 10.1016/j.colsurfb.2011.01.026. Epub 2011 Jan 26.
The attachment of Pseudomonas (P.) putida onto well (KGa-1) and poorly (KGa-2) crystallized kaolinite was investigated in this study. Batch experiments were carried out to determine the attachment isotherms of P. putida onto both types of kaolinite particles. The attachment process of P. putida onto KGa-1 and KGa-2 was adequately described by a Langmuir isotherm. Attenuated Total Reflection Fourier Transform Infrared Spectroscopy and Nuclear Magnetic Resonance were employed to study the attachment mechanisms of P. putida. Experimental results indicated that KGa-2 presented higher affinity and attachment capacity than KGa-1. It was shown that electrostatic interactions and clay mineral structural disorders can influence the attachment capacity of clay mineral particles.
本研究考察了假单胞菌（P. putida）在结晶良好（KGa-1）和结晶不良（KGa-2）高岭石上的附着情况。通过批量实验确定了 P. putida 在两种高岭石颗粒上的附着等温线。P. putida 附着在 KGa-1 和 KGa-2 上的过程可以用 Langmuir 等温式很好地描述。衰减全反射傅里叶变换红外光谱和核磁共振被用来研究 P. putida 的附着机制。实验结果表明，KGa-2 比 KGa-1 具有更高的亲和力和附着容量。结果表明，静电相互作用和粘土矿物结构紊乱会影响粘土矿物颗粒的附着容量。
