鸡疟原虫的免疫显性表面抗原存在于唾液腺子孢子和卵囊子孢子中。

The immunodominant surface antigen of Plasmodium gallinaceum is present in both the salivary gland and oocyst sporozoites.

Rocha E M, Correa-Oliveira R, Carneiro C R, Lopes J D, Krettli A U

Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil.

Braz J Med Biol Res. 1990;23(11):1117-25.

Abstract

Monoclonal antibodies (MAbs) against surface antigens of Plasmodium gallinaceum sporozoites, an avian malaria parasite, were produced using spleen cells from mice immunized with sporozoites from mosquito salivary glands (SGS) or from midguts containing oocysts (OoS). 2. All of the 15 MAbs tested (11 anti-SGS and 4 anti-OoS) reacted with SGS and OoS by indirect immunofluorescence and circumsporozoite precipitation reactions. Fourteen of these MAbs (11 anti-SGS and 3 anti-OoS) produced a Western blot (WB) pattern identical to that produced with serum from mice hyperimmunized with viable intact sporozoites. 3. All MAbs and the immune sera recognized only two polypeptide bands of approximate molecular weight 76 and 64 kDa. 4. No difference in the WB pattern was observed when 9- or 12-day SGS or OoS extracts were used as antigens in WB. This antigenic similarity was confirmed when the total protein extracts were visualized on silver-stained SDS-PAGE gel.

摘要

利用用来自蚊唾液腺（SGS）或含卵囊的中肠（OoS）的疟原虫子孢子免疫的小鼠脾细胞，制备了针对鸡疟原虫子孢子表面抗原的单克隆抗体（MAb）。2. 所测试的15种单克隆抗体（11种抗SGS和4种抗OoS）均通过间接免疫荧光和环子孢子沉淀反应与SGS和OoS发生反应。其中14种单克隆抗体（11种抗SGS和3种抗OoS）产生的蛋白质印迹（WB）图谱与用活的完整子孢子超免疫小鼠的血清产生的图谱相同。3. 所有单克隆抗体和免疫血清仅识别两条分子量约为76 kDa和64 kDa的多肽带。4. 当将9天或12天的SGS或OoS提取物用作WB中的抗原时，未观察到WB图谱有差异。当在银染SDS-PAGE凝胶上观察总蛋白提取物时，证实了这种抗原相似性。