Johnson C H, Nakashima H
Department of Biology, Vanderbilt University, Nashville, Tennessee 37235.
J Biol Rhythms. 1990 Summer;5(2):159-67. doi: 10.1177/074873049000500207.
Cycloheximide inhibits light-induced phase shifting of the circadian clock and protein synthesis in Neurospora. Light resetting is not inhibited in mutants whose protein synthesis is resistant to cycloheximide. When light and cycloheximide are presented together at various circadian phases, the final phase shift is always determined by cycloheximide. This dual-treatment phase response curve approach may be useful for other studies using pharmacological treatments to analyze clock pathways. Taken together, the results suggest that synthesis of a protein (or proteins) is involved in the phototransduction pathway of the circadian clock in Neurospora.
放线菌酮可抑制光诱导的脉孢菌生物钟的相位移动以及蛋白质合成。在蛋白质合成对放线菌酮具有抗性的突变体中，光重置不受抑制。当在不同的昼夜节律阶段同时给予光和放线菌酮时，最终的相位移动总是由放线菌酮决定。这种双重处理的相位响应曲线方法可能对其他使用药物处理来分析生物钟途径的研究有用。综合来看，结果表明一种（或多种）蛋白质的合成参与了脉孢菌生物钟的光转导途径。
