Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
Analyst. 2011 Apr 21;136(8):1580-5. doi: 10.1039/c0an00846j. Epub 2011 Feb 17.
A promising and highly sensitive electrochemiluminescence (ECL) sensor for the detection of Cu(2+) based on Cu(+)-catalyzed click reaction is described in this paper. Firstly, 1-azidoundecan-11-thiol was assembled on the Au electrode surface via a simple thiol-Au reaction, then the propargyl-functionalized Ru(bpy)(3)(2+)-doped SiO(2) nanoparticles (Ru-SNPs) ECL probe was covalently coupled on the electrode surfaces via click chemistry. Cu(+), the catalyst for click chemistry, is derived from the electrolytic reduction of Cu(2+)via the Bulk Electrolysis with coulometry (BE) technique and without any reductants. It is found that the ECL intensity detected from the electrode surface has a linear relationship with the logarithm of Cu(2+) concentration in the range of 1.0 × 10(-15) to 1.0 × 10(-11) M with a detection limit of 1.0 × 10(-16) M. Also, the method is highly specific even in the presence of high concentrations of other metal cations. It has been applied to detect trace Cu(2+) in complex samples (hepatoma cell) without sample treatment.
本文描述了一种基于 Cu(+)催化点击反应的用于检测 Cu(2+)的有前景且高灵敏度的电化学发光（ECL）传感器。首先，通过简单的硫醇-Au 反应将 1-叠氮十一烷-11-硫醇组装在 Au 电极表面上，然后通过点击化学将炔丙基功能化的 Ru(bpy)(3)(2+)-掺杂 SiO(2)纳米粒子（Ru-SNPs）ECL 探针共价偶联到电极表面上。Cu(+)是点击化学反应的催化剂，通过库仑分析法（BE）技术电解还原 Cu(2+)而无需任何还原剂得到。结果发现，从电极表面检测到的 ECL 强度与 Cu(2+)浓度的对数在 1.0×10(-15)到 1.0×10(-11) M 的范围内呈线性关系，检测限为 1.0×10(-16) M。此外，该方法即使在存在高浓度其他金属阳离子的情况下也具有高度特异性。该方法无需样品处理，已应用于检测复杂样品（肝癌细胞）中的痕量 Cu(2+)。
