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基于喹喔啉的荧光探针用于定量估计蛋白质结合部位极性的研究进展。

Development of a quinoxaline-based fluorescent probe for quantitative estimation of protein binding site polarity.

机构信息

Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8571, Japan.

出版信息

Chem Commun (Camb). 2011 Apr 7;47(13):3867-9. doi: 10.1039/c1cc10183h. Epub 2011 Feb 17.

DOI:10.1039/c1cc10183h
PMID:21331415
Abstract

The compound 2-[(1E)-2-(1H-pyrrol-2-yl)ethenyl]-quinoxaline (PQX) is a promising fluorescent chromophore for the estimation of protein binding site polarity, due to its full-color solvatochromic fluorescence. A linear relationship was obtained between the peak emission wavenumber and E(T)(N) (normalized solvent polarity). The BSA binding site polarity was estimated from the solvatochromic plot.

摘要

化合物 2-[(1E)-2-(1H-吡咯-2-基)乙烯基]喹喔啉 (PQX) 是一种很有前途的荧光生色团，可用于估计蛋白质结合部位的极性，因为它具有全色溶剂化荧光。在峰值发射波数和 E(T)(N)（归一化溶剂极性）之间得到了线性关系。从溶剂化曲线可以估计 BSA 结合部位的极性。

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