Department of Chemistry, Washington State University, Pullman, WA 99164, USA.
Int J Oncol. 2011 May;38(5):1349-55. doi: 10.3892/ijo.2011.946. Epub 2011 Feb 17.
Prostate-specific membrane antigen (PSMA), a well-known biomarker of prostate cancer, has also been found to be highly expressed in the neovasculature of multiple non-prostatic solid tumors. As a consequence, it has the potential to become a biomarker for tumor-associated vasculature. Herein, we describe an in vitro model for assessing PSMA expression associated with tube formation by primary human umbilical vein endothelial cells (HUVECs) cultured in Matrigel and induced by tumor-conditioned medium (TCM) derived from human breast cancer cells (MDA-MB-231). In contrast to vascular endothelial growth factor (VEGF)-containing endothelial cell medium, TCM induced higher expression of PSMA in HUVECs. The vessel-like tubes were detected by imaging with fluorescent PSMA inhibitors. Consequently, this in vitro model is expected to enable subsequent studies aimed at determining the role of PSMA in angiogenesis and factors that induce it.
前列腺特异性膜抗原（PSMA）是前列腺癌的一种著名生物标志物，也被发现高表达于多种非前列腺实体肿瘤的新生脉管系统中。因此，它有可能成为肿瘤相关脉管系统的生物标志物。本文中，我们描述了一种体外模型，用于评估 PSMA 在由肿瘤条件培养基（TCM）诱导的原代人脐静脉内皮细胞（HUVEC）在 Matrigel 中形成管状结构时的表达情况，TCM 来源于人乳腺癌细胞（MDA-MB-231）。与含有血管内皮生长因子（VEGF）的内皮细胞培养基相比，TCM 诱导 HUVEC 中 PSMA 的表达更高。用荧光 PSMA 抑制剂进行成像可检测到类似血管的管状结构。因此，该体外模型有望能够进行后续研究，以确定 PSMA 在血管生成中的作用以及诱导其表达的因素。
