Department of Physiology, Life Science and Biology Pharmacopedia Institution, Shenyang Pharmaceutical University, Shenyang, 110016, Liaoning Province, People's Republic of China.
Cell Mol Neurobiol. 2011 May;31(4):629-34. doi: 10.1007/s10571-011-9658-5. Epub 2011 Feb 18.
This study was performed to determine whether minoxidil sulfate (MS), a selective Adenosine 5'-triphosphate-sensitive potassium channel (K (ATP) channel) activator, has an effect on the expression of caveolin-1 in the rat's brain tumor tissue. Using a rat brain glioma (C6) model, we found that the expression of caveolin-1 protein at tumor sites was greatly increased after intracarotid infusion of MS at a dose of 30 μg/kg/min for 15, 30, and 60 min via Western blot analysis. And the peak value of the caveolin-1 expression was observed in rats with glioma after 15 min of MS perfusion, which was significantly attenuated by reactive oxygen species (ROS) scavenger (N-2-mercaptopropionyl glycine, MPG). In addition, MPG also significantly inhibited the increase of blood-brain tumor barrier (BTB) permeability which was induced by MS. This led to the conclusion that the MS-induced BTB permeability increase may be related to the accelerated formation of caveolin-1 protein, and could be mediated by ROS.
本研究旨在确定硫酸米诺地尔（MS），一种选择性的三磷酸腺苷敏感钾通道（KATP 通道）激活剂，是否对大鼠脑肿瘤组织中窖蛋白-1的表达有影响。通过大鼠脑胶质瘤（C6）模型，我们发现经颈内动脉内输注 MS（剂量为 30μg/kg/min）15、30 和 60min 后，Western blot 分析显示肿瘤部位的窖蛋白-1 蛋白表达显著增加。并且在 MS 灌注 15min 后，荷瘤大鼠的窖蛋白-1 表达达到峰值，而过氧化氢（ROS）清除剂（N-2-巯基丙酰甘氨酸，MPG）显著减弱了这种增加。此外，MPG 还显著抑制了 MS 诱导的血脑肿瘤屏障（BBTB）通透性增加。这表明 MS 诱导的 BTB 通透性增加可能与窖蛋白-1 蛋白的加速形成有关，并且可能由 ROS 介导。
