Department of Parasitology, Guiyang Medical College, Guiyang, China.
Parasitol Res. 2011 Sep;109(3):567-74. doi: 10.1007/s00436-011-2285-8. Epub 2011 Feb 18.
Two novel genes encoding lactate dehydrogenase A (LDHA) and B (LDHB) homologues, respectively, were identified from the cDNA libraries of adult Taenia solium (T. solium). The two deduced amino acid sequences both show more than 50% identity to the homologues for Danio rerio, Xenopus laevis, Schistosoma japonicum, Sus scrofa, Homo sapiens, et al. The identity of the amino acid sequence between TsLDHA and TsLDHB is 57.4%, and that of the nucleotide sequence is 61.5%. Recombinant TsLDHA homologue (rTsLDHA) and TsLDHB homologue (rTsLDHB) were expressed in Escherichia coli BL21/DE3 and purified. Though there were some differences in the sequence, the two LDH isozyme homologues show similarity in the conserved LDH domain, topological structure, primary immunological traits, localization on the tegument of T. solium adult, and partial physicochemical properties. The linear B-cell epitope analysis of TsLDHA and TsLDHB discovered a TsLDHA specific epitope. The purified rTsLDHA and rTsLDHB could be recognized by rat immuno-sera, serum from swine, or a patient infected with T. solium, respectively, but Western blot analysis showed cross-reactions, not only between these two LDH members but also with other common human tapeworms or helminths. The results suggested that the two LDH homologues are similar in the characteristics of LDH family, and they are not specific antigens for immunodiagnosis.
从猪带绦虫（Taenia solium）成虫 cDNA 文库中分别鉴定出两个编码乳酸脱氢酶 A（LDHA）和 B（LDHB）同工酶的新基因。这两个推导的氨基酸序列与斑马鱼、非洲爪蟾、日本血吸虫、猪、人等的同源物的相似度均超过 50%。TsLDHA 和 TsLDHB 的氨基酸序列同一性为 57.4%，核苷酸序列同一性为 61.5%。重组 TsLDHA 同工酶（rTsLDHA）和 TsLDHB 同工酶（rTsLDHB）在大肠杆菌 BL21/DE3 中表达并纯化。尽管在序列上存在一些差异，但这两种 LDH 同工酶同系物在保守的 LDH 结构域、拓扑结构、主要免疫特性、在猪带绦虫成虫表皮的定位以及部分理化性质方面表现出相似性。对 TsLDHA 和 TsLDHB 的线性 B 细胞表位分析发现了一个 TsLDHA 特异性表位。纯化的 rTsLDHA 和 rTsLDHB 可分别被大鼠免疫血清、猪血清或感染猪带绦虫的患者识别，但 Western blot 分析显示这些两个 LDH 成员之间以及与其他常见的人类带绦虫或寄生虫之间存在交叉反应。结果表明，这两个 LDH 同系物在 LDH 家族的特征上相似，它们不是免疫诊断的特异性抗原。
