siRNAs 对 HSV-1 噬斑形成和 RR mRNA 相对表达水平的影响。
Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA.
机构信息
Biomedicine research and development center of Jinan University, Guangdong, Guangzhou 510632, China.
出版信息
Virol Sin. 2011 Feb;26(1):40-6. doi: 10.1007/s12250-011-3162-9. Epub 2011 Feb 18.
Abstract
RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40, respectively. In this study, we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.
摘要
RNA 干扰 (RNAi) 是一种引入的小干扰 RNA (siRNA) 可以导致具有相同序列的 mRNA 特异性降解的过程。人单纯疱疹病毒 1 (HSV-1) RR 由 UL39 和 UL40 分别编码的两个不同的同源二聚体亚基组成。在这项研究中，我们应用了针对 HSV-1 的 UL39 和 UL40 基因的 siRNA。我们表明，合成的 siRNA 有效地、特异性地沉默了 UL39 和 UL40 mRNA 的表达，并抑制了 HSV-1 的复制。我们的工作为 RNAi 作为抑制 HSV-1 复制的遗传工具提供了新的可能性。
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