Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, South Korea.
Cell Biol Int. 2011 Jul;35(7):741-5. doi: 10.1042/CBI20100705.
Neurite outgrowth from endogenous or transplanted cells is important for neural regeneration following nerve tissue injury. Modified substrates often provide better environments for cell adhesion and neurite outgrowth. This study was conducted to determine if MWCNT (multiwalled carbon nanotube)-coated electrospun PLCL [poly (l-lactic acid-co-3-caprolactone)] nanofibres improved the neurite outgrowth of PC-12 cells. To accomplish this, two groups, PC-12 cells in either uncoated PLCL scaffolds or MWCNT-coated PLCL scaffolds were cultured for 9 days. MWCNT-coated PLCL scaffolds showed improved adhesion, proliferation and neurite outgrowth of PC-12 cells. These findings suggest that MWCNT-coated nanofibrous scaffolds may be an attractive platform for cell transplantation application in neural tissue engineering.
内源性或移植细胞的神经突生长对于神经组织损伤后的神经再生很重要。经过修饰的基底通常为细胞黏附和神经突生长提供更好的环境。本研究旨在确定 MWCNT（多壁碳纳米管）涂覆的静电纺丝 PLCL[聚（L-乳酸-co-3-己内酯）]纳米纤维是否能改善 PC-12 细胞的神经突生长。为了实现这一目标，将两组细胞分别培养在未涂覆的 PLCL 支架或 MWCNT 涂覆的 PLCL 支架中 9 天。MWCNT 涂覆的 PLCL 支架显示出对 PC-12 细胞的黏附、增殖和神经突生长的改善。这些发现表明，MWCNT 涂覆的纳米纤维支架可能是细胞移植在神经组织工程中应用的有吸引力的平台。
