Howat D, Desa F, Chander C, Moore A, Willoughby D
Department of Experimental Pathology, Medical College, St Bartholomew's Hospital, London, U.K.
J Lipid Mediat. 1990;2 Suppl:S143-9.
The breakdown of rat articular cartilage has been studied in vivo and in vitro to observe the effect of platelet-activating factor (PAF) and interleukin-1 alpha (IL-1 alpha) on the net loss of glycosaminoglycan (GAG). When PAF was infused over a 2-week period into rats, there was no increased breakdown of implanted, cotton-wrapped, femoral head cartilage. However, there was a loss of proteoglycan from the femoral head cartilage of the recipient rats (native cartilage). This loss from the native cartilage was not apparent if the animal had not been implanted with cartilage. Using an in vitro system, the effect of PAF and IL-1 alpha on rat femoral head cartilage was further examined. Separately, neither agent had any significant effect on GAG content. However, when combined, there was a significant loss of GAG. The significance of these results is discussed.
已在体内和体外研究了大鼠关节软骨的分解情况，以观察血小板活化因子（PAF）和白细胞介素-1α（IL-1α）对糖胺聚糖（GAG）净损失的影响。当在2周的时间内将PAF注入大鼠体内时，植入的、用棉包裹的股骨头软骨的分解没有增加。然而，受体大鼠（天然软骨）的股骨头软骨中蛋白聚糖有所损失。如果动物没有植入软骨，这种天然软骨的损失并不明显。使用体外系统，进一步研究了PAF和IL-1α对大鼠股骨头软骨的影响。单独使用时，这两种物质对GAG含量均无显著影响。然而，当两者联合使用时，GAG出现了显著损失。讨论了这些结果的意义。
