Research Laboratory LR99ES11, Department of Biochemistry, Tunisia.
Exp Mol Pathol. 2011 Jun;90(3):276-9. doi: 10.1016/j.yexmp.2011.02.004. Epub 2011 Feb 17.
Recent findings suggest that inflammation plays a role in atherosclerosis and its acute complications. Several known mechanisms may play at least a partial role in this process. One of the most likely mechanisms involves lipopolysaccharide (LPS) and its receptor, CD14. The C(-260)T single nucleotide polymorphism (rs2569190) in the promoter region of the CD14 receptor gene has been reported to be associated with a higher risk of MI. Others studies, however, have not corroborated these findings. Considering the contradictory results, the aim of the present study was to investigate the possible association between the CD14 C(-260)T polymorphism and the risk of MI in the Tunisian population. A total of 321 Tunisian patients with MI and 344 healthy controls were included in the study. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. The frequency of TT homozygous genotype for the CD14 C(-260)T polymorphism was 26.2% in MI patients and 27.0% in the control group. However, the genotype distribution and allele frequencies were not significantly different between MI and controls subjects. Moreover, the odds ratio for MI associated with the TT genotype failed to reach statistical significance (OR=1.22; 95% CI: 0.85-1.77; p=0.272). These results do not support the hypothesis that the C-260T polymorphism of CD14 gene contributes to the genetic susceptibility to MI in the Tunisian population studied.
最近的研究结果表明，炎症在动脉粥样硬化及其急性并发症中起作用。几个已知的机制可能至少在这个过程中起部分作用。其中一个最可能的机制涉及脂多糖（LPS）及其受体 CD14。已经报道 CD14 受体基因启动子区域的 C(-260)T 单核苷酸多态性（rs2569190）与 MI 的风险增加有关。然而，其他研究并未证实这些发现。考虑到结果相互矛盾，本研究旨在探讨 CD14 C(-260)T 多态性与突尼斯人群 MI 风险之间的可能关联。共纳入 321 例突尼斯 MI 患者和 344 例健康对照者。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析进行基因分型。MI 患者 CD14 C(-260)T 多态性 TT 纯合基因型的频率为 26.2%，对照组为 27.0%。然而，MI 和对照组之间的基因型分布和等位基因频率没有显著差异。此外，与 TT 基因型相关的 MI 的优势比未能达到统计学意义（OR=1.22；95%CI：0.85-1.77；p=0.272）。这些结果不支持 CD14 基因 C-260T 多态性导致研究中突尼斯人群 MI 遗传易感性的假设。
