Department of Paediatric Dentistry, Institute of Stomatology, Nanjing University Medical School, 30 Zhongyang Road, Nanjing 210008, China.
J Dent. 2011 Apr;39(4):332-9. doi: 10.1016/j.jdent.2011.02.004. Epub 2011 Feb 17.
To evaluate the influence of chemomechanical caries removal on bond strengths of three adhesive systems to caries-affected human dentine.
45 extracted human third molars with occlusal moderate caries were used. One half of each cavity was prepared with chemomechanical method and the other half with bur as the control. Three adhesive systems, Prime&Bond NT (Dentsply, Germany) without any previous conditioning, an etch-and-rinse adhesive (One-Step, Bisco, USA) and a self-etching adhesive (Adper Prompt-L-Pop, 3M ESPE, USA), were applied. The compomer or resin crowns were, then, built up. The prepared specimens were sectioned for scanning electron microscopy (SEM) examination and microtensile bond strength (MTBS) testing. Data were analysed using two-way ANOVA with subsequent application of Student-Newman-Keuls test at p<0.05.
Opened dentinal tubules and less smear layer were visible by SEM after chemomechanical caries removal, whilst an obvious smear layer covering the dentine surface with occluded tubules was exhibited after bur excavation. Resin tags were seen only in specimens with chemomechanical excavation when Prime&Bond NT system was applied. Statistical analysis of the values of MTBS failed to show significant difference between caries removal methods. However, the values of MTBS for One-Step system achieved with two caries removal methods were both significant higher than other adhesive systems.
Chemomechanical caries removal did not influence the bond strengths of the adhesive systems used in this study to caries-affected human dentine. Highest bond strength was achieved with application of etch-and-rinse adhesive system.
评估化学机械去龋对三种黏接系统与龋损牙本质黏接强度的影响。
选取 45 颗具有咬合面中度龋的人第三磨牙。每个窝洞的一半用化学机械法制备，另一半用涡轮钻作为对照。应用三种黏接系统：Prime&Bond NT（德国登士柏）不做任何预处理、酸蚀-冲洗黏接剂（One-Step，美国碧兰）和自酸蚀黏接剂（Adper Prompt-L-Pop，3M ESPE，美国）。然后，构建复合树脂或树脂冠。制备好的标本进行扫描电子显微镜（SEM）检查和微拉伸黏接强度（MTBS）测试。使用双因素方差分析（ANOVA），随后采用 Student-Newman-Keuls 检验，p<0.05 时差异有统计学意义。
SEM 下观察到化学机械去龋后牙本质小管张开，玷污层较少；而涡轮钻去龋后牙本质表面有明显的玷污层覆盖，小管封闭。当应用 Prime&Bond NT 系统时，仅在化学机械去龋的标本中可见树脂突。MTBS 值的统计分析显示，两种去龋方法之间无显著差异。然而，两种去龋方法下 One-Step 系统的 MTBS 值均显著高于其他黏接系统。
化学机械去龋方法不影响本研究中应用于龋损牙本质的黏接系统的黏接强度。应用酸蚀-冲洗黏接系统可获得最高的黏接强度。
